Study On Isolation And Identification Of Lactic Acid Bacteria From S.Guianensis Sw. And Application For Silage Preparation

In order to investigate the effect of lactic acid bacteria on Stylosanthes guianensis Sw. silages characteristics, four lactic acid bacteria strains were separated from the Stylosanihes SW. of Hainan proVInce, which were identified bacterially using the classical classification and sequences of16S rDNA. The results showed that four strains were Enterococcus hirae, Enterococcus faecalis, Lactococcus lactis subsp. Hordniae and Lactobacillus paraplantarum. The capability of lactic acid production of HN113and HN91were better than HN78and HN87. HN113strains have the most powerful acid yield, which can quickly access into the logarithmic growth field, and HN113strains which could be suitable for development as makers of microbiological ecological silage fermentation preparations is the Homofermentative LAB.In this experiment, forage was assigned to8treatments:S.guianensis Sw.'Reyan No.2: no additive(Ⅰ),5%molasses (Ⅱ), HN113(Ⅲ)and5%molasses&HN113(Ⅳ); S.guianensis Sw.'Reyan No.5:no additive(V),5%molasses (Ⅵ), HN113(Ⅶ) and5%molasses&HN113(Ⅷ).By comparing and research, we got the conclusionas as follows:each treatment group of Stylosanthes2and Stylosanthes5had the same fermentation quality trends.The results showed that teatment of (Ⅳ&Ⅶ) groups'pH value decreased fast and the number of lactic acid bacteria increased after silage compared with the control, the inhibited actiⅥties on Aerobic bacteria and yeasts were better than the control group, the pH value of5%molasses&HN113(Ⅳ) and5%molasses&HN113(Ⅷ) are3.75and3.87, they are the best of all. The results of chemical analysis indicated the content of Ammonia nitrogen,Neutral Detergent Fiber and Neutral Detergent Fiber of5%molasses&HN113groups were significantly lower than the control group(P<0.01). Lactic acid, Dry Matter, Crude Protein and In Ⅵtro Dry Matter Digestibility content of5%molasses&FIN113groups were obVIously higher than the control group (P<0.01).The ash content in each group no significant difference (P<0.05). Butyric acid was not found in the3treatment groups.The silage quality of5%molasses&HN113(Ⅳ&Ⅵ) groups were better than the treatment of5%molasses (Ⅱ&Ⅵ).All of the above, the quality of S.guianensis Sw. silages was improved significantly with the contribution sugar and Lactobacillus paraplantarum (HN113) which separated from S.guianensis Sw..