Studies On The Relativity Between The Activity Of Na+, K+- Atpase And Oocytes In Vitro Maturation In Yanbian Yellow Cattle

The purpose of this article is to study the character of oocytes in Yanbian Yellow Cattle by researching the effects of temperature on oocytes of Yanbian Yellow Cattle and the max concentration of ouabain, the specific inhibitor of Na+, K+-ATPase, and the effects of granular cell on the active of Na+, K+-ATPase, which are the great foundation of the further study about the relationship between the active of Na+, K+-ATPase and the vitro mature of oocytes of Yanbian Yellow Cattle, and the relativity between the activity of Na+, K+-ATPase and oocytes in vitro mature. The activity of oocytes from different grade was detected, oocytes of Grade A and Grade E were cultured respectively in vitro for Oh,6h,12h,and 24h, and then their Na+, K+-ATPase were determined and compared with each other, the activity of Na+, K+-ATPase of oocytes in vitro mature was compared with that of oocytes in vivo mature. The results showed that the active of Na+, K+-ATPase reached the max value at 50℃, and the active degraded below or over 50℃. The partheno-oocytes cann't cleavage completely in present of ouabain at 2 mmol/L. The active of Na+, K+-ATPase in grade A group, ova nuda group and hyper-mature group were detected, there were no significant difference among the three groups tested, but the value of ova nuda group was highest. The activity of Na+, K+-ATPase of oocytes of Grade E was the highest in the three groups, Grade A, Grade E and Grade F, however, without significant statistics difference. The activity of Na+, K+-ATPase of Grade A oocytes cultured for Oh,6h,12h and 24h had an increased trend without significant statistics difference. But that of Grade E oocytes cultured for 24 h was significantly higher than 0 h,6 h and 12 h. The activity of Na+, K+-ATPase of oocytes in vitro mature was significantly higher than that of oocytes in vivo mature and immature oocytes in vitro mature.The conclusions were that the optimal react temperature of Na+, K+-ATPase of the oocytes of Yanbian Yellow Cattle was 50℃and the max suppressible concentration of ouabain was 2 mmol/L. The active of Na+, K+-ATPase in ova nuda group was highest. Oocytes in different grade had different activity of Na+, K+-ATPase, and had an increased trend in the process of mature. Oocytes in vitro mature had higher activity of Na+, K+-ATPase.