Studies On Serum-free Mature Medium Of Somatic Cell Cloning In Yanbian Yellow Cattle

The use of serum-free medium for oocyte maturation embryo culture in vitro and the development, although in recent years it was constantly trying, but there is still some places to improve. Experiments were compared different concentration of BFF, PVA, PVP and BSA on the impact of development the cattle oocytes in vitro maturation rate,cleavage rate,4-6 C development,8-16 C development rate,morula and blastocyst rate:1,In the different concentration of BFF to oocyte matured in vitro and in the development rate experiment, when 10%BFF the rate of maturation, the cleavage rate, 4-6 cell development rate and 8-16 cell development rate higher than other groups, demonstrated that 10%BFF is the best concentration.2,In the BFF is inactivated in vitro oocyte maturation and growth rate of experiments, they are no difference in maturation rate, But on the cleavage rate the difference is significant. As can be seen from the conclusions, although BFF can replace serum, but inactivated as serum is not necessary.3,The effect of different concentration of PVA in the oocyte maturation and embryo development in vitro, the experiment measured: In maturation rate of 1.0mg/ml is highest , but no difference with 1.5mg/ml, The best early development rate is 1.0 mg/ml, higher than other groups. Add PVA more than 1.5 mg/ml, cleavage, but they are abnormal embryos, cleavage rate is lower than non-supplemented group. The results showed that 1.5 mg/ml of PVA is in vitro maturation of oocytes of the highest concentration.4,After determining the best concentration of BFF and PVA , combination of both. The results showed that: a combination of both adding is better than alone.5,The effect of different concentration of PVP in the oocyte maturation and embryo development in vitro, the experiment measured: 0.3%group is better than other groups from maturatal rate and developmental rate , therefore suitable for maturation oocytes in vitro development of PVP and the best concentration is 0. 3%.6,The effect of different concentration of BSA in oocyte maturation and embryo development in vitro, the experiment measured: 0.4%group is better than other groups from maturatal rate and developmental rate ,but the concentration of BSA has nothing to do with maturation. therefore suitable for maturation oocytes in vitro development of BSA and the best concentration is 0.4%.7,After comparing of four serum-free mature medium, we concluded that: On maturation rate, all groups of liquid have no difference, they can replace serum for oocyte maturation; On cleavage and development rate, 10%BFF+PVA has no diference,it can replace serum, so it is the most suitable matrational liquid to replace serum.