| Green plants play an important role in urban ecological environment. They can release large amount of volatile organic compounds(VOCs), which have certain influence on atmospheric environment, air quality, human physical and mental health. Therefore, it's important to thoroughly and systematically research the components and variation rules of plant VOCs,the impact of human intervention on the release of plant VOCs and the health functions of plant VOCs, all of which can provide scientific theory for configuring and managing reasonable woods, forming healthy forest that is good for human health. Based on above, Pinus bungeana Zucc.,one of the most widespread conifers in North China is chosen for research. By collecting volatiles gas with headspace sampling method and analyzing gas sample with ATD-GC/MS, we investigate the composition, diurnal variation and temporal pattern of volatile emission after mechnical damage. Besides, the health function of Pinus bungeana Zucc. is evaluated by animal ethological method. Results are showed as follows:1,The Pinus bungeana Zucc. could emit 130 kinds of compounds, including alkane, alkene, alkyne, alcohol, ketone, aldehyde, ester, acid, aromatic hydrocarbon and ether, etc. It could be seen that terpene is the mainly VOCs from occurrence frequency and relative concentration. The compounds which had higher relative concentration were a-pinene and D-limonene.2,The diurnal variation curve of TVOCs was"three peaks, three valleys(W+V)", the higher concentration time appeared at 13:00, 19:00 and 23:00, the lower concentration was at 3:00, 15:00 and 21:00. Overall, it seemed that the concentration of TVOCs in the day is greater than that of night.3,The relative concentration change of alkene in the whole day was not evident. Its highest concentration time appeared at 19:00 and lowest value appeared at 9:00; The relative concentration of alkane fluctuated evidently, looking like a wavy curve, whose highest concentration time appeared at 3:00, lowest appeared at 5:00. 4,Among the VOCs in Pinus bungeana Zucc., terpenes were the mainly VOCs that are good for health, and the bad ones were alkyl chloride and aliphatic aldehydes whose number of C are below 8. The total concentration of VOCs that favors for health was large throughout the day and much higher than the VOCs concentration which was bad for health.5,The Pinus bungeana Zucc. did not release new compouds after mechanical damage. As same as the control group, its main compounds contained alkene, ketone, alcohol, aromatic. However, compared with the control group, the mechanical damaged group showed a great change in the propotion of each components: the relative concentration of alkene increased obviously in the early stages of injury, the relative concentration of ketone and alcohol increased obviously in the latter stages of injury.6,The relative concentration of TVOCs which has advantage for health did no change after mechanical damage, but its composition varied with time. In addition, the relative concentration of TVOCs which has disadvange for health decreased obviously.7,The mouse had some bearing threshold on volatile odor released by Pinus bungeana. After being treated of the Pinus bungeana VOCs, the mouse's excitement increased, exploration and cognitive enhanced. In addition, this change was positively correlated with concentration, to some extent. However, the exicitability, exploration and cognitive ability of mice was inhibited if the concentration exceeds its threshold. |
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