| The biological activity of six antivirus chemical agents currently used in production was tested by the half a leaf withered spot method. The result showed that all six kinds of chemical agents have a low inhibitory ratio to Tomato spotted wilt tospovirus (TSWV). Among the six kinds of agents, Ningnanmycin has relatively higher activity against TSWV, with 57.60% inhibitory ratio under 1000 times dose, with 87.53% inhibitory ratio in vivo passivation, and with 74.77% inhibitory ratio in vivo protection. And Ningnanmycin has a good effect against the extension of TSWV. The result of pharmacodynamic test on the system host showed that Kangdufeng Pesticides, Dongwang Pesticides and Ningnanmycin have inhibitory ratio 54.78%, 50.47% and 49.15%, respectively.On the basia of the above findings, active substance of botanical anti-TSWV was screened in this study. The inhibitory effect of crude extract of 8 kind plants to TSWV was also tested by the half a leaf withered spot method. There were more than 50% inhibitory ratio in three kinds of crude extracts, and the inhibitory ratio in crude extracts of Pokeberry Root and spinach was 94.81% and 92.63%, respectively. The biochemistry mechanism of the Pokeberry Root and spinach resistance to TSWV was preliminary studied. The result revealed that:when the Garden Petunia was inoculated with TSWV and then treated with Pokeberry Root and spinach, there were some relationships among the enzymic activity of PAL,SOD,POD,PPO,the content of chlorophyl and regulation substance. The increase of resistance-related enzymic activity was obviously inclued, and the content of chlorophyl rise in Pokeberry Root. |
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