Characterization And Expression Of Barley HvPrP1 Gene During Seed Development And Under Abiotic Stress

Based on gene cloning, gene sequences encoding HvPrpl protein was isolated from barley for the first time. Real-Time Quantitative RT-PCR and macroarray were used to analysis the expression pattern of HvPrpl genes from barley, rice and Arabidopsis. The results were outlined as following.1. The putative HvPrpl gene sequences from barley was 3909bp, encoding 857 amino acids. The nucleotide sequences of HvPrpl gene shared 78% and 87% sequence similarity with those from Arabidopsis and rice, respectively. The amino acids sequence of HvPrpl protein is similar to the rice Prpl protein and the Arabidopsis Prpl protein, with similarities of 94% and 79%. Domain analysis revealed that the putative HvPrpl protein of barley has all the conserved domains of the HvPrpl protein family, including a highly conserved N-terminal domain and some TPR and HAT at the C-terminal.2. The expression pattern of putative HvPrpl gene from barley was assayed with gene specific primers by Real-Time Quantitative RT-PCR. Putative HvPrpl gene is expressed in all the four examined organs including stem, leaf, root and seeds at different stages of development. The results showed that, interestingly, without environment impact, the HvPrpl gene expression is stable at all tissue except the seeds in 18 and 20 days after flowering. Within 72h following cold stress, the HvPrpl gene expressive level has significant difference. The Prpl gene from different plant has uniform expression pattern in difference physiological period and abiotic stresses was deduced according to the comparison results of macroarray data from barley, rice and Arabidopsis. Promoters predict found that 53 TFBses were share by three promoters. Further analysis found that have 32 TFBses are stress-relation, which contain 3 of which are drought-relational,1 of which are cold-relational,10 of which are light-dependent,4 kinds are induction by hormone,5 kinds are hold by storage protein,2 of which are salt-relation and the cold-relation, storage protein-relation TFBses are functional.3. These results indicated that different plant Prp1 proteins may play the same role as that in fission yeast. It plays a regular role during spliceosome activation and cell cycle.