Application Research Of Liquor Electrical Resonance Sensor In Diagnosis Of Avian Influenza

Avian flu (avian influenza) is caused by A-type influenza A virus avian infectious diseases. In recent years, several studies have shown that this virus can infect humans cause disease, called avian influenza. Avian flu to humans has brought huge economic losses and health threats. According to the China Animal Health and Epidemiology Center, Room comprehensive analysis of international veterinary matters data published in 2010, many of the world informed of major animal epidemic occurred, the bird flu in the first row, which indicates that the current outbreak of avian flu remains a serious still requires a lot of manpower and resources, inhibit or alleviate the disease.The traditional avian flu detection methods, such as hemagglutination and hemagglutination inhibition, less demanding on the equipment, the economy, low cost, but the proficiency of the operator demanding, especially for the analysis and determine the results, unlike other countries such as microplate reader judge the outcome of such a visual and mechanical detection of the nucleic acid used in the instrument, although accurate but most expensive, is not appropriate to do a lot of promotion at the grassroots level.Liquor Electrical Resonance Sensor in the diagnosis of avian influenza on the instrument the application of research to the traditional method of hemagglutination and hemagglutination inhibition test production compared to the establishment of a new bird flu detection techniques and the development of new detection equipment, to achieve a low The costs can be convenient for the purpose of detection of avian influenza, can be used in the inspection and quarantine departments, epidemic prevention departments and hospitals. Liquor Electrical Resonance Sensor with the detection of avian flu rapid test, economic, and sensitivity advantages. Moreover, detection of the reaction used in accordance with the procedure chips after washing, reusable, suitable for large-scale testing. Once the outbreak can be effectively detected.The research of liquid electric resonance sensor technology is avian influenza detected by a biological sensor technology, a kind of instrument, the principle is the use of antigen specificity and antibody response resonant quartz transducer and the surface quality of the load of the height variation sensitivity of the combination of biochemical reactions occur by measuring the resonant frequency changes liquids, to characterize the results of a novel piezoelectric immunosensor detection methods.First, the subject of embryo culture method used to test a large number of standard drugs used for the proliferation of AIV, AIV virus by about 70ml of allantoic fluid, and then red blood cells using formaldehyde adsorption of the virus was purified by two viruses, tube 1 about 6ml, tube 2 about 5ml, calculated by UV spectrophotometry at the same time that the concentration of tube 1 is 18.31mg/ml, tube 2 concentration 15.35mg/ml.Will mature hemagglutination and hemagglutination inhibition test and compare the liquid electric resonance experiment, when tested in the virus titer dilution range, and its resonant frequency is less than the minimum 300000Hz, liquids Measuring resonant sensor The mean frequency shift value and its natural logarithm of dilution is linear, and linear correlation coefficient R2 value close to 1, ruled hemagglutination positive. When the analyte is lower than the dilution of the virus titer, its resonant frequency is greater than 300000 Hz, while the average frequency shift value and its dilution of the linear relationship between the natural logarithm of a large deviation, the fluid under test at this time hemagglutination titer ruled negative.