Distribution Of Interferon In Hormone Cells Of Goat's Adenohypophysis

There are various secretion cells in adenohypophysis which can regulate animal reprodu- ctive.IFN-αand IFN-γimmunoreactive substance was widely localized in adenohy -pophysis, which can regulate neuroendocrine function and plays an important physiological function in HPO axis. Related research of IFN-γhas been done by our laboratory already, But not found more correlational research report under different physiologic period ,also not found some special issue about IFN-α.Therefore,the model of Goat in different physiological period was established, and the method of immunohistoch emical ultrasensitive SP was used to investigate the expression and distributing variety of IFN-αand IFN-γimmunoreacti- ve substance in adenohypophysis under different physiological period and distributing in hormonal cells in pituitary and expressive relation with PRL,FSH,LH. The main results were as follows:1,The distributing and express quantity of IFN-αand IFN-γin adenohypophysis under gestation.IFN-αand IFN-γimmunoreactive substance was widely localized in adenohypoph- ysis under different gestation and mainly localized in cytoplasm and cell membrane ,also exist complete cell tinting partially. Early in pregnancy,IFN-αwas distributed in acidophilic cell and basophilic cell of distal portion and pars tuberalis.IFN-γmainly localized in chromophobe cell of pars tuberalis.IFN-αexpression was higher than IFN-γ(P<0.05); in cyophoric metaph- ase,IFN-αand IFN-γpositive cells significantly reduced(P<0.05) than prophase , and mainly localized in pars tuberalis,except IFN-αwas expressed in partes intermedia partially. IFN-αexpression was significantly higher than the IFN-γ(P<0.01); In cyophoric anaphase, IFN-αand IFN-γexpression was significantly ascend and mainly localized around blood sinus.IFN-αimmunoreactive substance expression was higher than IFN-γ(P<0.05).The result indicate that with the gestation period change , distribution of IFN-αand IFN-γwill appear difference, exist significant difference .2,The distributing and express quantity of IFN-αand IFN-γin adenohypophysis under oestrum.IFN-αand IFN-γimmunoreactive substance was expressed strongly in adenohypop- hy -sis under different oestrum .IFN-αand IFN-γmainly localized in distal portion and pars tuberous in adenohypophysis under different oestrum.More positive cells that immunoreactive substance localized in membrane were found under oestrous metaphase. positive cells dispers- e distribution under anaphase and immunoreactive substance distinct decrease.Compare the express quantity of positive cells:IFN-αexpression was higher than IFN-γcells under oestrum prophase (P<0.05);IFN-αexpression was higher than IFN-γcells under oestrum prophase (P<0.05); while under oestrum metaphase the IFN-αexpression was very significant(P<0.01) compared with the IFN-γpositive cells;IFN-αexpression reduced (P<0.05) when oestrum metaphase compared with the IFN-γpositive cells;Dioestrum,the number of IFN-αpositive cells greater than IFN-γcells(P>0.05),but there are no significant differences.Indicate that distributing and expression of IFN-αand IFN-γwas affected by oestrum.3,Compared expression between IFN-α, IFN-γand PRL,FSH,LH under gestation oestrum.The expression of IFN-αwas higher than PRL under gestation (P<0.01),while the expression of IFN-γ, FSH and LH was significantly decreased(P<0.05).IFN-αmainly localized in in prolactin cells and gonadotrophs and positive cells of IFN-αless than PRL(P<0.05),while greater than FSH and LH cells(P>0.05).Gestation,IFN-γmainly localized in gonadotrophs and positive correlation with FSH;positive cells of IFN-γless than PRL, FSH and LH(P<0.05);Oestrum,positive cells of IFN-α,IFN-γand PRL greater than FSH and LH(P<0.05).IFN-αlocalized in prolactin cells while IFN-γmainly localized in gonadotro- phs.expression of IFN-αsimilar to PRL cells,But there is a significantly difference compared with LH (P < 0.05).expression of IFN-γsimilar to LH cells, But there is a signifi- cantly difference compared with FSH (P < 0.05).Result show that hypophyseal hormone and IFN-αand IFN-γwas changed along with the physiologic period . According to the research results, expression of IFN-αand IFN-γwas affected by physiologic period in adenohypophysis; hormonal level of PRL,FSH and LH could be regulate by IFN-αand IFN-γ.Speculate, there is a positive or negative correlation effect on secretion,synthesis and release of reproductive hormones.