Pigs Infected With Mycoplasma Haemphilus Animal Model Of The East

Pig bloodthirsty mycoplasma (haemosuis) also say pig Mycoplasmal Eperythrozoon filaments (suis with red is parasitic in pigs) and red surface, plasma and free from bone marrow and cause to piglet fever, anemia, jaundice and sow the symptom such as abortion is the characteristic of pathogenic microorganisms. Pig bloodthirsty mycoplasma disease often mixed with other pig disease prevention and cure difficult occur, clinical, give pig industry caused the financial meltdown. But so far, domestic and international against the disease etiology, epidemiology, immunology research also insufficiently thorough, so as not to make effective prevention and control measures. Therefore, it is quite necessary to set up a proper and stable pig bloodthirsty animal mycoplasma infection, so as to further understand model pig bloodthirsty mycoplasma lay the foundation.This study to promoting agricultural science research institute for cultivating Oriental sweet pig experimental animals with injections of dexamethasone, to reduce their immunity, then method with intraperitoneal injection mode vaccinated pigs bloodthirsty mycoplasma, to establish a bloodthirsty mycoplasma infection eastern incense pig animal model. Through the clinical symptoms observation, blood pressure drops specimens microscopy, routine blood test and ELIS A,PCR pathogens such as means of antibody detection method of eastern incense pigs were infected with the animal model for identification. The results show that affects pigs bloodthirsty mycoplasma 14d or so, A, B, C, D group of eastern incense pig successively show temperature increases, breathing and heartbeat to accelerate, visual mucosal pale, yellow dye, angular and other typical clinical symptoms; Artificially infected bloodthirsty mycoplasma, A, B, C three groups of incense in after infection pig 7d article 5 - red reached 80%, and the infection rates have 12d 9 - red up to 90%, after the first 14d rates decreased, but has remained at 75% group D, and eastern incense pig bloodthirsty mycoplasma infection, sexual present Oriental sweet pig group E no change; Group in eastern incense pig pig bloodthirsty mycoplasma infection, leukocytes significantly increased after total (P<0.01), lymphocyte number, RBC hemoglobin content of total, are significantly (P<0.05), red blood cell olume significantly (P< 0.01); Artificially infected pigs bloodthirsty mycoplasma the first 3d PCR detection after A, B and C into three groups of incense in the blood of pig have bloodthirsty mycoplasma infection, group D exist when the first PCR detection to bloodthirsty 5d mycoplasma; Indirect ELISA antibody test shows that, A, B, C three groups of eastern incense pig levels of antibodies in infection of the highest 7d sweet pig group D antibody levels when 21 d from 3d until that have risen, eastern incense will affect low immunity antibody levels rise pig. The research has successfully established a bloodthirsty TiDong FangXiang pigs were infected with the mycoplasma animal model for pigs, bloodthirsty mycoplasma disease research laid a foundation.