Preliminary Research On The Identification Of The Pathogen、Diagnosis And Vaccine On Mycoplasma. Bovis Pneumonia

In the wordwide, the Mycoplasma bovis has been a most importment pathogen of the cattle feedlot. It could not only cause a series of symptoms such as pneumonia, arthritis, mastitis, conjunctivitis and so on, also with other pathogens in the period. Since the first Mycoplasma bovis was isolated from a cattle with mastitis in 1961, it have been spred to every corner of the world becase of the transport. Then it caused a huge spread of the pneumonia to every cournries, took a huge economic loss, got a great threat to animal husbandry.Our subject mainly studys on three aspects:the identification of the pathogen on the Mycoplasma bovis pneumonia; the cloning and expression of the protein GAPDH, building a I-ELISA way to survey the serology epidemic on Mycoplasma bovis pneumonia; the research on the inactive vaccine of the Mycoplasma bovis pneumonia. The more details as below:1. The identification of the pathogen on the Mycoplasma bovis pneumonia occured in China.From the 2008, there were many reports about the epidemic situation with the respiratory tract signs like the Contagious bovine pleuropneumonia from the cows which transported from other provinces to Hubei. The subject studied according to isolate and culture the pathogen, then observed the "fried-egg like" colonies in the microcrope, and blast the sequence of the 16S rRNA PCR with the 16S rRNA sequence in the Genebank, the similar was 99%. We went on the artificially infected experiments, and found the same the clinical signs and isolated the same pagthogen. From the result, we successfully identificated the pathogen is the Mycoplasma bovis, named it Mycoplasma bovis pneumonia, and first published the the epidemiology of the Mycoplasma bovis pneumonia in China.2. The cloning and expression of the GAPDH, building an I-ELISA way to survey the serology epidemic on Mycoplasma bovis pneumonia. There are many reports about the Mycoplasma bovis gene GAPDH as a potential value to identificate the pathogen of the Mycoplasma bovis pneumonia, but less related news in domestic. The main aim of our study is to utilize the genetic engineering to cloning and expression the GAPDH gene, building anâ… -ELISA way, to use for the serology epidemic in clinical trails.We detected 41 health samples and 57 disease samples. In the disease samples, the positive percents was 78.97%, negative part was 8.77% and the doubtful was 12.28%, it was according with the practical feedlot. So the protein GAPDH as a way to detect the mycoplasma bovis, it was more reliability, more easy, short period, and maybe as a potential way to detect the samples.3. The research on the Mycoplasma bovis inactive vaccine.We packet the 36 cows in 3 groups randomly, and used different adjuvant vaccine immune 3 times, then stimulated with the Mycoplasma bovis through the trachea. Comparing of cilincal signs, antibody rise and fall level, fluctuation of temperature and weigh in different groups, we concluded that the ISCOM as a adjuvant to immune can stimulated the body get a high antibody litre, decreased the clinical signs, and avoided the loss weigh. So it was a potential adjuvant for the mycoplasma bovis pneumonia inactive vaccine for more research and clinical practice.