Expression Analysis Of Dehydrin In 4 Genotypes Wheat Under Soil Drought Stress

Most unfavorable natural environments are bad for plant's growth and development, and even cause plant's death when they get worse. Drought is one of common environmental stresses, as well as a important factor to limit agricultural production. Wheat (Triticum aestivum L) is one of the most important crops around world, and it always has large-scale massive losses for the annual drought environment, threatening the quality of mankind life. Thus, the entire world devotes a great deal of strength to wheat drought resistance research, so as to resolve this scabrous problem which can improve food production and quality and the level of human life.Dehydrin is one of stress response proteins, it palys a key role in plant adapation and resistance to stress. Although much progress has been made in dehydrin`s structure, location, function and gene expression regulation research up to now, there still has a long way to go to wheat dehydrin exploration. Also because short-term stress experiment is give little role to the actual application, we conducted this study. Our objective is want to through the experiment to investigate the law of dehydrin expression in different genotypes wheat at various developmental stage under drought stress. The results will provide theoretical bases to the study of wheat drought stress response mechanism, and to answer how wheat dehydrin resist drought stress. All of that are important to wheat transgenic molecular breeding and improve crop drought resistance.The methods we used are include this: Plant four different drought resistance wheat for materials(Drought sensitive type:zhengyin1# and Low drought tolerance7532; Drought tolerance type:Shanhe6# and High drought tolerance86211) in pots, impose natural drying stress for drought stress treatment to the four genotypes wheat at the stage of seedling, tillering, jointing and flowering, use the techniques of SDS-PAGE and Western blot to detect the law of dehydrin expression.Though the above methods we get following results: There were no dehydrin detected in the four genotypes wheat without drought stress, while dehydrins with the molecular weight of 45KDa and 37KDa were detected at the four developmental stage in the all wheat under drought stress, and 49KDa dehydrin only expressed in the High drought tolerance wheat, 40KDa dehydrin only expressed in the Low drought tolerance wheat, 28KDa dehydrin only expressed at the specific developmental stage of four genotypes wheat. The laws of dehydrin expression in drought tolerance type wheat are as follows: dehydrin has little expression at the early stage of drought stress, as the stress increasing, the dehydrin express volume increase quickly and reach the peak under the worst stress, and the express volume decreased quickly after rehydration. The laws of dehydrin expression in drought sensitive type wheat are as follows: dehydrin has much expression at the early stage of drought stress, as the stress increasing, the dehrdrin express volume increase slowly after a slightly reducing, and reach the peak at the first day of rehydration, after that the amount of dehydrin decreased slowly in this type wheat.By analyzing the results we reach the conclusion are as follows:1. Expression of some dehydrin proteins in 4 genotypes Wheat has genetic diversity, while others have the characteristics of generality.2. The expression of dehydrin are different at the various developmental stage in the same genotype wheat.3. All of the dehydrins are accumulated under the drought stress and decrease under the rehydration, this suggests that dehydrin express is closely related to the drought stress.4. The expression pattern of dehydrin are different between drought tolerance type and drought sensitive type wheat. First, the accumulation in sensitive type is less than the tolerance type under the same level drought stress. Besides, the tolerance type wheat is sensitive to worse drought stress, however, the sensitive type wheat is sensitive to drought stress and the ability of rehydration recover is weaker than the tolerance type.