Isolation And Characterization Of Microsatellite Markers In Pelteobagrus Fulvidraco And Genetic Diversity Analysis Of Populations In The Middle Of Yangtze River

Yellow catfish (Pelteobagrus fulvidraco) is regarded as a good candidate for freshwater culture in China. We isolated nine microsatellite markers from Pelteobagrus fulvidraco genomic DNA libraries. These nine novel dinucleotide microsatellite loci will provide markers of sufficient power to detect population structure in P. fulvidraco. Polymorphism was tested using 36 individuals from DT population. The observed number of alleles ranged from 3 to 8. The ranges of observed and expected heterozygosity were 0.3056 to 0.9444 and 0.5129 to 0.8435 respectively, the average of PIC was 0.6296. The result showed that the nine microsatellite loci will be suitable for genetic analysis.We then used six microsatellite DNA markers to investigate the genetic diversity of P. fulvidraco from four wild populations. A total of 153 individual yellow catfishes were collected in four geographic locations in the Yangtze River:Dongting Lake (DT), Poyang Lake (PY), Chang Lake (CA) and Chong Lake (CO) and a total of 55 alleles were identified from the six loci with high PIC value of each loci(PIC=0.6693-0.8018). All the four wild populations in the Yangtze River showed moderate allelic (Ne,=3.885-5.907; Na=5.333-8.333) and high gene diversity (Ho=0.633-0.773;He=0.727-0.838). Among the 24 population loci,14 showed significant deviation from the Hardy-Weinberg equilibrium. Cluster analysis using UPGMA revealed a close genetic relationship of P. fulvidraco between Chang Lake and Chong Lake while the population from Dongting Lake and Poyang Lake diverged. FST values showed significant genetic differentiation among three populations (DT, PY and CA) and that CA and CO can be treated as one population. The information on the genetic diversity in different geographic populations and population structure of P. fulvidraco in the Yangtze River obtained in this study is useful for setting up suitable guidelines for future genetic improvement by selective breeding.