| Chinese Angelica is umbrelliferae plant. Angelica root can be used as medicine , Angelica hepatic is help for heart, spleen. Medicine property is sweet, acrid, warm.It can enrich the blood, regulate menstruation pain, dryness and other effects. Angelica is one of the most frequently used traditional medicine. Many scholars of the chemical composition of Angelica, pharmacology and clinical applications are for a more detailed study. Isolated from this plant phthalide, terpenoids, aromatic compounds and other ingredients. We received a lot of active ingredients and the knowledge of its Indication. In this paper, the different growing areas within the Angelica Minxian major pharmaceutical active ingredients in the volatile oil were analyzed comparative study. Direct steam distillation (HD method) to extract the essential oil of Angelica. Single factor and orthogonal experimental design were optimized by steam distillation of volatile oil of the optimum conditions. Using gas chromatography - mass spectrometry (GC-MS) on the steam distillation of different planting sites Minxian volatile oil in the identification of chemical constituents were separated. The peak area normalization method for the determination of the relative content of each component, and comparative analysis of different points of the Angelica plant essential oil extraction and chemical composition differences. The results show that:1.In the steam distillation of volatile oil in the process, the extraction time (D) is the most important factors on the volatile oil yield of a highly significant (P <0.01); Medicine crushing degree (A) and immersion time (B) is a secondary factor, the impact of volatile oil extraction have significantly (P <0.05), Medicine crushing degree (A) greater than the impact of immersion time (C). Volatile oil extraction rate of factor D> A> C> B, the extraction time> medicine crushing degree> soaking time> liquid ratio.2.Volatile oil extraction under the optimal conditions for the principle of determining the optimal volatile oil extraction process is A2B1C2D3. 60 degrees head crushed herbs, solid to liquid ratio of 1:8, soaking time 4 h, 8 h of steam distillation.3.Optimal use of volatile oil extraction process on the territory of five different planting.Minxian points volatile oil was extracted, yield different.Qingshui town: 0.96%;Xizhai town: 1.08%;Shili town: 0.84%;Minyang town: 1.22%;Qinxu town: 0.86%.4.Detected by chemical oscillating system components of Angelica sinensis in Minxian different regions aqueous extract of origin characteristics:in Minxian different regions, Angelica sinensis aqueous extract chemical oscillation period and amplitude curves were significantly different.5.Chemical composition of volatile oil determined by GC-MS. Comparative analysis of the main component, different collection of volatile oil components to significantly different. The main chemical components of the relative percentage differences are significant. The main component of volatile oil in Qingshui town: Benzene phthalate butene, Z-ligustilide, different key enol Eucalyptus oil, 6 - diene butyl 1,4-cycloheptane, and the relative percentage of 66.8%. The main component of volatile oil in Xizhai town: Benzene phthalate butene, Z-key ligustilide ,[-]- Eucalyptus oil enolase, is fourth alkanol, and the relative percentage content of 86.7%. The main component of volatile oil in Shili town: Benzene phthalate butene, Z-ligustilide, flowers Eucalyptus orientalis en ,[-]- key oil enolase, and the relative percentage content of 81.5%.The main component of volatile oil in Minyang town: Benzene phthalate butene, Z-ligustilide are fourteen key Eucalyptus oil alkanols ,[-]- enolase, and the relative percentage content of 83.5%.The main component of volatile oil in Qinxu town: Benzene phthalate butene, Z-key ligustilide ,[-]- Eucalyptus oil enolase, is fourth alkanol, and the relative percentage content of 87.7%. |
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