Studies On Insecticidal Active Constituents From The Seeds Of P. Orientale L.

P.orientale L. is an annual herbs belonging to the family polygonum .The mature seeds of the p.orientale L. have been used in Chinese folk medicine for the treatment of stomach-ache, intumescene, inflammation,bad stomach digest. Scholars have taken the attention of the P.orientale L. since the twentieth century. But few chemical constituents and bioactivities of the Seeds of P.orentale L. have been reported. In order to research the chemical constituents and bioactivities of P.orientale L. We have studied the constituents of the Seeds of P.orientale L.Study on bioactivities of extracts from the seeds of P.orientale L. against Mythimn separate (Walker) was carried on by bioassay-driven separation. The result showed that insecticide active composition in seeds of P.orientale L. were present in the ethyl acetate fraction from seeds. B2, B3, B5, B6, B15 were more contact poison activity than others, After ethyl acetate partition was isolated in the silica gel column.This thesis describes the phytochemical investigation of the fraction of ethyl acetate from seeds of P.orientale L. 13 compounds were isolated by means of column chromatography(CC), preparative thin layer chromatography(PTLC), and recrystallization etc. They are G1-G13. B2 isolated from the fractions G1, G2, G3 and G4. B3 isolated from the fractions G5, G6, G7,G8, G9, G10, and G11. B5-B6 isolated from the fractions G12. B15 isolated from the fractions G13.In combination with the spectroscopic data from literature, the structures of 9 compounds have been elucidated on the basis of comprehensive spectroscopic methods, including UV, ¹H-NMR and ¹³C-NMR etc. They are Linoleic acid, 2, 3-dihydroxypropyl palmitate, Ethy hexadecanoate, 1, 3-dilinolenin, n-Nonanol, 3-hydroxypropy-12-ene, 6-hydroxy-7-methoxycinnamic acid, Stigmasterol, hexadecanol,β-sitosterol. We found a new bioactive compounds, prepare for it apply for patent.