Research On Distribution And Diversity Of Ammonia-Oxidizing Archaea In Different Soil Environment

Ammonia-oxidizing archaeal can process ammonia oxidizing in an environment that has a very low oxygen concentration and its amoA gene has much more copy number than AOB does. This has attracted more concern than ever before. In this study, the molecular biology technology has been used for the study of diversity composition and phylogent of the uncultured AOA, cooperating with the Bioinformatics software MEGA AND Mothur. By the studing of environment factors that affect the distribution of AOA, and the abundance and richness of AOA, an experimental supporting has been provide to discover the mechanistic of the AOA Metabolism.To extract environment sample metagenome, and retrieve amoA gene we used the uncultured methods of molecular biology technology. Environment samples were recovered from upland wild soil, river bank wetland, water sediments, artificial wetland, agriculture soil, paddy soil. AOA amoA gene specific primers were used to retrieve AOA amoA gene from samples. Then Muther and MEGA were used for OTU definition and abundance diversity computing. Results were used for analyzing.The result shows that AOA has a wide distribution and diversity in a variety of environment and is tolerant for the changing of the environmental factors. There are different dominant species in wetland and upland soil. It can be inferred that some kind of wetland AOA may have more tolerance for they are wide distributed in many kinds of environment, so that in the latter researches environment samples should be chosen for different applications. The diversity of the AOA is also varied between diferent environments. The additional nutrient added artificially leads to more richness of the species, indicates the respond of AOA to the nutients. However AOB seems have an opposite reaction to the nutrients application. AOA has a domainate position compared with AOB in these environment samples.