The Research Of Microorganism Composition Responces To Environment Conditions With The Next Generation High Throughput DNA Sequencing Thchnology

Soil microorganisms are an important part of soil, and play an important role in the soil nutrient cycling and sustainable soil quality development. Currently, lots of researches are studying the disturbance of anthropogenic activity on microorganism diversity. However, most of them adopted the traditional molecular finger print technology with low resolution. Such techniques could not reflect the effects of environment condition on the numerical-less while functional dominant microorganisms. In this study, we taking16SrRNA gene as molecular target and adopted the next generation454pyrosequencing to investigate the effect of land use changes on the diversity of microorganisms, and the effect of different altitude on microorganism community composition. The effect of land use change and different altitude on ammonia oxidizing microorganism was also investigated. At the same time we adopted the454pyrosequencing technology to investigate the ammonia oxidizing microorganisms of treatment of13CO2and13CO2+C2H2after DNA-SIP incubation for28days. Our results are as follows:(1) After the convertion from the native desert to100-year farmland,27-year farmland,33-year Pinus forest,28-year Poplar forest and21-year shrub land, we found that the microorganisms which proportion>4.37%in native desert were suppressed, while the microorganisms which proportion<4.37%were promoted. The land use change was the most important factor that affected the community composition.(2) The microorganism community compositions on the Qilian Mountain at different altitudes were investigated with the desert soil at1900m altitude as control (CK). We found that the dominant microorganisms in the soils of Qilian Mountain were Proteobacteria, Actinobacteria and Acidobacteria, while the dominant microorganisms in the CK were Proteobacteria, Actinobacteria and Firmicutes; The microorganism compositions were affected by the soil properties associated with the altitude of Qilian Mountain.The dominant soil ammonia oxidizing microorganisms were also affected by soil properties associated with altitude of Qilian Mountain. The abundance of ammonia oxidizing archaea (AOA) was higher than the ammonia oxidizing bacteria (AOB) at different altitudes of Qilian Mountain; The relative abundance of AOA decreased, while the relative abundance of AOB increased with the altitude. (3) Results from the28-day incubations of13CO2and13CO2+C2H2treatment indicated that the AOB and nitrite oxidizing bacteria (NOB) were the dominant ammonia oxidizing microorganisms in the Fengqiu agricultural soil, while the AOA was less important than the AOB and NOB.