| The soil of massoniana plantation in Zijinshan Forest of Hunan Hengyang was the mainly research object of the study. We used both biochemistry activity experiments indoor and related mathematical analysis to research the activity indices of soil microbial in rhizosphere and un-rhizosphere at different ages, different seasons, different soil. The indices have soil bacteria, fungi, actinomyces, azotobacter, phosphorus bacteria, potassium bacteria, soil dehydrogenase, catalase, urease, microbial biomass C, N and basal respiration, matrix induction respiration, soil nutrient factor. Discuss the soil quality of microbiology indicators of different plantation age massoniana plantation to build soil health evaluation index system. In order to provide the reference for analysis in southern China massoniana plantation soil ecological function. The results were as followed:1. In the five samples of study, numbers of three kinds of soil microbial was bacteria more> fungi>actinomyces. Microbiological physiology class in vertical distribution character was azotobacter> phosphorus bacteria> potassium bacteria. The number of microorganism in soil profile has similar vertical distribution. Decreased as soil depth increase. In 0-20cm, there was the most, then reduced sharply. As plantation age growing the number of different kinds of microbe change in a "low-high-low". Its seasonal change is more consistent and obvious, it performed for July> October> March> January (summer> autumn> spring> winter). The number of microorganism in rhizosphere was more than it in un-rhizosphere, that was R> S.2. The vertical change characteristics in soil. Change of soil enzyme activity, soil microbial biomass and Soil respiration were identified, which was that those decrease with the increase of the soil depth, i.e.0-20cm> 20-40cm> 40-60cm> 60-80cm.3. Along with the change of season, in addition to soil urease active peak appeared in October, the minimum appeared in January. The other two enzymes soil, soil microbial biomass and soil respiration displaied the same on the seasonal variation. which of peak appeared in July, the minimum appeared in January.4. On the change characteristics of rhizosphere and non- rhizosphere soil, soil enzyme activity, soil microbial biomass and Soil respiration display all the same, the rhizosphere soil was higher than the non- rhizosphere soil, the change characteristic that be performing R>S.5. The vertical change characteristics of soil nutrient factors were the same as the display of soil enzyme activity, soil microbial biomass and Soil respiration. And on the change characteristics of R and S, which the change characteristics of rhizosphere and non- rhizosphere soil was R>S. About the pH value was increase with the increase of the soil depth, the value pH in rhizosphere soil was lower than the non-rhizosphere soil's. the pH value of the Pinus massoniana forest soil during the higher acidity and the low acidity. The lowest was 3.85, which belonged to the higher acidity, the minimum was just 5.94, which belong to the low acidity. the pH value was decrease with the increase of the stand ages.6. Based on analysising the correlation between microbial index, it showed that there was extreme significant positive correlations among the number of microorganism, enzyme, microbial biomass and respiratory function except phosphorus bacteria. Additional moisture content and pH were not relevant. This showed that there was close relationships among number of microorganism, enzyme activity, microbial biomass, respiratory function and organic matter, organic C, rapidly-available N and P of nutrient factors.7. In this study, the system of soil biological evaluating indicators that was based by applying method of statistical analysis. The first principal component Yl mainly reflected the variables of microbial activity index which contains bacteria, fungi, total microbes, azotobacter, phosphorus bacteria, minerals bacteria, dehydrogenase, urease, microbial biomass C, microbial biomass N, basal respiration, stromal induction respiration, organic matter, rapidly-available N and P. It accounted for 75.815% and played important roles. The second principal component Y2 mainly reflected actinomyces, the actinomycetes, catalase, organic C, total N, total P, moisture content, pH indicators microorganism activity such as integrated variables, its total to 20.181% accounted for, has certain effect, but less influence of evaluation. Use soil integrated fertility index (IFI) as final evaluation standard of soil, the result showed that soil biological characteristics has obvious difference in different stand ages Pinus massoniana forest, along with the growth of Pinus massoniana forest's stand ages, its change trend began gradually increase from young forest, when was near-mature forest arrived at the peak, then after nearly mature forest, its distribution began to decline, the tendency of changes are relatively obvious. |