AFLP Analysis Of Rice With Aerial-Root And High-Pole

Large number of variants materials were obtained by far fate sub-hybridization transferring genomic DNA fragments of maize into the receptor of rice. In our study,two kinds of variants materials of rice HN-28 and HN-31 as the research materials(Aerial-root and high-pole),using AFLP of molecular maker technology for polymorphism analysis.then, analyzing the different fragments after recycling,cloning and sequencing by Bioinformatics.Polymorphism analysis of the genomic DNA of 2 kinds of rice variation strain and its controls with 64 pairs selective primers of AFLP molecular maker technology,18 pairs optimum selective primers were screened.We found that after the DNA of maize transferred into the receptor of rice, that compared with maize the similarity of genome DNA in variation lines was higher than that of negative control. There were 91 and 90 DNA polymorphic bands in variation line HN-28 and HN-31 which there were 49 and 31 new bands, 25 and 45 absent bands, 17 and 14 target bands, reached the polymorphic ratio of 7.8%. and 8.2% . The similarity ratio of amplified bands in the variation lines HN-28, HN-31 with the negative control (Rice) were 88.2% and 90.8%, The result showed that between the variation lines HN-28 and HN-31 with the negative control (Rice) had significant differences. The similarity ratio of amplified bands in the variation lines HN-28, HN-31 and the negative control (Rice) with the positive control (maize) were 32.5%, 30.4% and 26.5%, The result showed that the similar rate between variation strains and maize controll were higher than the similar rate between rice controll and maize controll. It is show that the DNA fragments of maize may transferred into the receptor of rice by far fate sub-hybridization.Analysed the target fragments with BLAST, there were 16 bases matched 100% between the target fragment that from P4M8 (P4M8-HN28 and P4M8-HN31) with the fragments of maize controll, but we can't find any high matching rice sequence and high matching maize sequence. There were 4 target fragments (P5M3-HN28, P5M3-HN31, P5M8-HN28 and P5M8-HN31) can find some matching rice sequence with many bases differences. The differency ratio is 7.3%, 7.3%, 12.5% and 12.5%. There were 2 target fragments (P5M7-HN28 and P8M5-HN28) can find individual bases differences. The differency ratio is 0.8% and 0.9%. We surmise that the character may in relation to base mutation It is also may be the DNA fragments of maize transferred into the receptor of rice by far fate sub-hybridization.Analysed the new fragments with BLAST, The result showed that there were there were 3 new fragments (P3M6-HN28, P3M6-HN31 and P5M8-HN31) can find some matching rice sequence individual bases differences The differency ratio is 1.7%, 1.5% and 1.2%. New fragments P4M5-HN28 can find some matching rice sequence with many bases differences . The differency ratio is 14.3%.The cause was may the bases of rice genomic DNA sequence happened mutations by far fate sub-hybridization transferring DNA fragments of maize into the receptor of rice. Protein function changed as the sequence was changed. It impacted the character of variation strain expressed.