Analysis Of Gut Bacteria Diversity From Rice Planthoppers

This study is mainly about intestinal bacterium of the white-backed planthopper[Sogatella furcifera (Horvath)] and brown planthopper [Nilaparvala lugens (Stal)]. We initially studied the intestinal microorganism of white-backed planthooper and brown planthopper by traditional and molecular gene anlysis methods. and studied the physiology and biochemistry characteristic of bacterium we got from planthopper.①We got 11 genuses bacterium from white-backed planthopper and brown planthopper by 16S rRNA molecular gene anlysis method, they were Wolbachia t.Brevundimonas sp. Azospirillum sp, Ochrobactrum sp. Herbaspirillum sp, Comamonas sp. Acinelobacter sp. Stenotrophomonas sp. Leucobaler sp,. Cardium. TM7 bacterium.②Designing primers according to gene sequence that we have identified, and to detect the infection rate of every bacterium in laboratory colonies and farm colonies. The results show that there is a little difference intersubject laboratory and farm colonies of white-backed planthopper and brown planthopper. and there is a great difference between laboratory and farm colonies. Wolbachia and Cardium had high infection rate in the white-backed planthopper from laboreatory and farm colonies:Acinelobacter and TM7 bacterium had high infection rate in brown planthopper from laboratory and farm colonies.③We isolated 10 genuses bacterium ang one uncultured bacterium from white-backed planthopper and brown planthopper by traditional cultural method, they were Bacillus sp.Chryseobacrerium sp, Staphylococcus sp, Microbacterium sp, Actinobacterium sp, Brevundimonas sp. Rhodococcus sp. Acinetobacter sp. Enterobacter sp, Erwinia sp. We got Bacillus sp., Chryseobacterium sp.. Staphylococcus sp., Microbacterium sp., Actinobacterium sp., Brevundimonas sp., Rhodococcus sp.,Acinetobacter sp and one uncultured bacterium from white-backed planthopper. and we got Bacillus sp..Chryseobacterium sp.. Actinobacterium sp., Microbacterium sp..Enterobacter sp.,Erwinia sp from brown planthopper.④We have studied the cultivatied character, the staining reaction and physiology and biochemistry reaction of cultivatied bacterium. We have initially knowed these bacterium.⑤We isolated each bacteria from laboratory imidacloprid screening population, buprofezin screening population of white kacked planthopper and fiproni screening population of brown planthopper. Each of the three strains can grow by imidacloprid. buprofezin and fiproni as sole carbon source and energy source.