Study Of Helicoverpa Armigera (Hübner) Receptor For Activated C Kinase 1 Gene

Receptor for activated C kinase 1 (Rackl) belongs to a large family of WD 40 repeat proteins and is a highly conserved protein found in both eukaryote and prokaryote(Smith et al.,2000). At first,Rack1 was considered as a repceptor of protein kinase C(PKC)in mammals(Ron et al.,1994).But now, the role of RACK1 as a scaffold protein in a lot of signalling processes(chen et al.,2006). Cotton bollworm, Helicoverpa armigera(Hübner), is a world pest, cause huge damage to a lot of crops. In this experiment, the characterizations of receptor for activated C kinase 1 gene from Helicoverpa armigera (Hübner) were studied and these studies further provide foundation to understand the insect response to adversity, The main results are as follows:1. Receptor for activated C kinase 1 (Rackl)cDNA was isolated by using reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE).Nucleotide sequence analysis revealed that open reading frame (ORF) of Rack1 has 957 bp in size encoded 319 amino acid residues. 3'untranslated region (UTR) of Rack1 contains 112 nucleic acids and 5'UTR contain 36 nucleic acids.2. The temporal expression of Rack1 transcript was studied by using Real-time PCR. The results showed that Rack1 was up-regulated in molting stage,It imply that Rack1 maybe concrol the molting stage.3. The Rack1 expression analysis after hormones challange was studied by using Real- time PCR. The results showed that Rack1 was up-regulated by molting hormone (20e) and inhibited by juvenile hormone analogue and starvation.4. The Rack1 expression analysis after hunger was studied by using Real- time PCR. The results showed that Rack1 was inhibited by hunger.