Transgenic Rice For Nicosulfuron Tolerance

Nicosulfuron is sulfonylurea herbicide. It is an ultra-efficient, broad-spectrum postemergence corn herbicide, and widely used for control of annual grass weeds, broadleaf weeds and some Cyperaceae in Maize fields. Nicosulfuron can be easily absorbed in plant roots, leaves, and is transported in the xylem and phloem. Nicosulfuron is an inhibitor of acetolactare synthase(ALS), which is a key Enzyme for the biosynthesis of all the branched-chain amino acid, valine, isoleucine, and leucine. Nicosulfuron is a widely used herbicide since the early 90's.Five possible P450 genes were selected by bioinformatic analysis of the genomic sequences of the maize inbred line B73. All the 5 genes were transformed into rice by Agribacterium-medicated transformation. The transformed rice events from one gene, name 253, were found to be tolerant to nicosulfuron. From field trials of nicosulfuron, showed that all 253 transgenic rice events can grow well within the normal nicrosulfuron herbicide use concentration(80mg/L) of com, part of the transgenic rice events of nicosulfuron resistance level can reach 640mg/L.This study created nicosulfuron tolerant transgenic rice. We believe these transgenic rice could be utilized for weed control mangerment for rice field.