Selective Cytotoxicity And Mode Of Action Of Actinomycetes SSPRC-612243 On Insect Cell Lines

An Actinomycete was found in the bioactive selectiong process, which can produce insecticidal activity substances named SSPRC-612243. This paper intend to explore the mode of action of SSPRC-612243 on different animal cell lines, by cell biology and insect immunology methods including emulsification characteristics, insect immunity, cell immunity characteristics, sensitivity,apoptosis and membrane potential of Tn5B1-4, HEK293 and HepG2 cell lines. Meanwhile, we used traditional pesticides such as chlorpyrifos, imidacloprid, chlorfluazuron, hexaflumuron, fenoxycarb, tebufenozide, chlorfenapyr, fipronil, and avermectin stimulate cells, preliminarily proved SSPRC-612243 could impact insect cell lines mitochondria, interfere with cell energy metabolism, which different from traditional pesticides.The experiments of different cell lines impacted by SSPRC-612243 and tween 80 showed that the cell viability of the Tn5B1-4 was increased with up-regulating concentration of tween 80 when exposure to low concentration. But with high concentration, it decreased. The result of SSPRC-612243 on insect hemocytes immunity system in vitro, showed the number and phagocytosis rates of Plasmatocyte and Granulocyte were declined,and The NOS activity of hemocytes was maintaine activated with the increasing of SSPRC-612243 concentration, in time-dose manner.. So SSPRC-612243 is a good insect immunity system inhibitor.The result of SSPRC-612243 on the cell apoptosis and mitochondrial membrane potential of Tn5B1-4,HEK293 and HepG2 cell lines showed that there is no inhibition for HEK293 and HepG2 cell lines, while conversely, it exhibits highly selective toxicity for Tn5B1-4 cell with durative and reversible properties.And the results of flow cytometer analysis shown SSPRC-612243 can change the mitochondrial membrane potential to induce cells apoptosis. And the Ca2+ synergy is important with the cytotxicity of SSPRC-612243.