| Tetranychus urticae Koch is a worldwide temperate and subtropical regions widely distributed spider mites, but also the flowers on one of the important pest mites, host plants of 200 species of 50 families. This Tetranychus urticae Koch with piercing-sucking mouthparts taking leaves of plants in the sap, threatening the plants flower buds, leaves and stems, the plants began to dry out the affected leaves, to the incalculable loss of China's agricultural economy. The paper by mushroom collection, purification train to get mushroom mycelium slope, and centrifuged through mushroom products, broken product, crude toxin toxicity screening, selection has a significant effect of mushroom poisoning Amanita leopard spots and ring shank mushroom brown scales. Mushroom products through centrifugation, broken product, the toxicity of crude toxin screening for different processing of the two spotted spider mite poison test. Statistical results are as follows:(1) fromMay 2010, October 2011 collecting Large fungi. The fungi collected species have 38 species, including poisonous fungus 6 kinds, edible fungus 32 species. Choose 6 major macrofungi as selected bacteria.(2) On June 19, 2010 in Shenyang bird fleas collected from the market, to capture the total number of 32, including 30 female adults, male adults 7. Male and female ratio is about 4.28:1. The capture of Tetranychus urticae Koch feeding laboratory, inoculated soybean leaves. Total of about 900 ~ 1200, reached the required number of mites..(3) Through the 18 major mushroom centrifugal liquid on Tetranychus urticae Koch as mites and larva of the poison test, we can see leopard spots virus Amanita ring shank with brown scales on Tetranychus urticae mushroom poisoning effect of 71.6%, respectively, cytotoxicity was 88.3%. Choice of these two cell disruption mushroom liquid, toxic crude as the test source to do further testing. Two types of mushroom extract and identification of bacteria for toxin was Leopard type of hallucinogenic drug Amanita mushroom toxin composition does not contain toxic umbrella peptide; brown scales while loop handle toxic mushroom umbrella peptides and peptide two kinds of toxic substances. Leopard rundlet crude toxin toxicity test for spider mites in the first 12h, 24h, when LC50 were 0.120,0.075 g / ml, the concentration of 0.03,0.19 g / ml; LT50 were 35.064d, 7.783d; umbrella brown scales crude toxins within 24h lethal concentration LC50 were 0.134,0.0628 g / mL, Tetranychus urticae Koch in the time of death in 0.01,0.17 g / mL concentrations of LT50 were 112.136d, 8.173d.(4) For ultra-low temperature of Mushroom centrifugation, cell disruption, organic extraction solvent to extract the crude toxin of Tetranychus urticae with the same concentration of 0.07g/ml different bioassay methods kill Tetranychus urticae Koch. However, multiple large gap between the required liquid. Required for mushroom slide dipping acaricide 25-35ml, Leopard umbrella, brown umbrella scales corrected mortality were 35%, 83.5%; slide to be capillary liquid 0.1ml, Leopard umbrella, brown scales rundlet corrected mortality were 36.5%, 86.5%; leaf liquid immersion method to be 20-25ml, leopard spotted umbrella, brown umbrella scales corrected mortality were 43.5%, 91.5%. Slide in the standard test used under the impregnation, the slide can be used under laboratory conditions, the capillary method, field trials can be toxic effects leaves impregnation.(5) Strain aging effect on the mites have a significant impact. 0.07g/ml Leopard poisonous Amanita same concentrationⅣ,Ⅹbehalf of the strains obtained crude toxins kill mites on the leaves were 51.5%, 43.5%; 0.015g/ml ring shank mushroom brown scales the same concentrationⅣ,ⅹgeneration of toxic strains obtained crude Tetranychus urticae Koch kill rate of 46.5%, 26.5%, significantly different. |
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