Replication Of Southern Rice Black-streaked Dwarf Virus In Its Insect Vector Sogatella Furcifera

Southern rice black-streaked dwarf virus (SRBSDV) caused severe damage onrice production since2008. SRBSDV belongs to the genus Fijivirus in the family ofReoviridae. This rice virus is transmitted by its insect vector, Sogatella furcifera in apersistent-propagative manner. However, the study of replication and transmission ofSRBSDV in the body of Sogatella furcifera was unknown. To control this virus, wehave to interference with transmitted way of the virus by insect vector, which wasbased on the study of replication and transmission of SRBSDV in the vector.This paper studied the replication and transmission of SRBSDV in the insect andits cultured cells. After dissecting the Sogatella furcifera infected with SRBSDV, weused fluorescent antibody of virus to detect the virus infection byimmunofluorescence. The nonstructural proteins P9-1and P7-1of SRBSDVaccumulated in the midgut epithelium and salivary gland, which indicated thatSRBSDV could replicate in the midgut and salivary gland of insect vector. In theelectron micrographs of thin sections of salivary gland, viroplasm that is the site forviral replication and tubular structures that contain virus particles could clearly beobserved. It was clear that SRBSDV could replicate in the body of Sogatella furcifera.We used the extraction of SRBSDV from diseased rice plants to infect cultured cells.Then RT-PCR and Western blot assays were used to test viral infection in culturedcells. Immunofluorescence showed that the structural protein P10and nonstructuralproteins P9-1and P7-1could be accumulated in the infected cells. The electronmicrographs of thin sections of infected cells showed that viroplasm and tubularstructures could be observed. P9-1distributed on the matrix of viroplasm, and P7-1distributed on the surface of tubule which enclosed virus particles.In conclusion, SRBSDV could replicate in the body of Sogatella furcifera and itscultured cells.