| As Nox2is the downsteam effector protein of Rac1, it could conclude that Rac1regulate the concentration of superoxide in hypha and conidiospore cells. In this way,Nox2may server in the infection-dependened signalling pathway. To make surewhether the proteins interacted with Nox2are involved in the infection-dependenedsignalling pathway. In this study, we demonstrated whether four proteins, MoHmt1(Mgg04584), MoLpe (Mgg05968), MoUf (Mgg06000) and MoZts(Mgg06374)interacte with Nox2in vivo by yeast two-hybrid analysis. The four proteins wereobtained through yeast two-hybrid screening.In translational level, the results of yeast two-hybrid showed that the fourproteins did not interact with Nox2in vivo. In transcriptional level, we tested therelative expression quantity of the target genes betweenâ–³nox2mutant and wild typeby real time PCR. The results showed that the MoLpe was up regulated, MoZts wasdown regulated,but MoUf and MoHmt1were no abviously changed. According tothe bioinformatical analysis, we identified genetic relationship of the four proteins inMagnaporthe oryzae with other fungi in organic evolution. Here, we also got geneticanalysis of the four genes through separately constructing knock-out mutants. Theseresults showedâ–³Mohmt1mutant was defective in hyphal morphogenesis anddecreased pathogenicity moderately. However, compared with wild type,â–³Molpemutant,â–³Mouf mutant,â–³Mozts mutant were unchanged in hyphal morphogenesis,spores quantity and pathogenicity.Altogeher, our data may point toward a role for MoHmt1in mycelialdevelopment process and pathogenicity. But the depletion of MoLpe, MoUf orMoZts does not affect development process and pathogenicity. |
