Inlfuence Of Transgenic Rice With A Cry1Ab Gene From Bacillus Thuringiensis On Sogatella Furcifera

In this paper, influence of transgenic rice with a cry1Ab gene from Bacillusthuringiensis on Sogatella furcifera was evaluated systematically from three parts: theobservation of growth and development, the detection of enzyme activity and Bt toxinprotein content with the method of feeding Sogatella furcifera on transgenic rice witha cry1Ab gene from Bacillus thuringiensis and non-transgenic parental rice varietyMinghui86continuously and biochemical detection. The result of indoor feeding,observation and biochemical detection which continued three generations showed thattransgenic rice with a cry1Ab gene from Bacillus thuringiensis had a certain influenceon Sogatella furcifera,but the influence was not significant in general. Specificconclusions were as follows:The influence of transgenic rice with a cry1Ab gene from Bacillus thuringiensison the growth and development of Sogatella furcifera was not significant in general.The survival rate of Sogatella furcifera nymph and adults fed on transgenic rice with acry1Ab gene from Bacillus thuringiensis remained stable in general, while thesurvival rate of Sogatella furcifera nymph and adults fed on Minghui86showedobvious downward trend with the growth of age. There was no significant differenceof the development period of egg to third instar nymph between Sogatella furciferafed on transgenic rice with a cry1Ab gene from Bacillus thuringiensis and Minghui86,but the former was slightly shorter than the latter.The influence of transgenic rice with a cry1Ab gene from Bacillus thuringiensison enzyme activity of Sogatella furcifera adults and aging nymph was not significant,and host factor and generation factor both had an influence on enzyme activity ofSogatella furcifera adults and aging nymph. The result of detection of five kinds ofenzyme activity (catalase, CAT; peroxidase, POD; superoxide dismutase, SOD; glutathionetransferase, GST; acetylcholinesterase, AchE) of Sogatella furcifera adults and aging nymphfed on transgenic rice with a cry1Ab gene from Bacillus thuringiensis and Minghui86which continued three generations showed that enzyme activity of Sogatella furciferaadults and aging nymph fed on transgenic rice with a cry1Ab gene from Bacillusthuringiensis did not increase or decrease continuously, existing fluctuation amonggenerations. The double factors variance analysis results showed that host andgenerational interaction were the main effect which caused the change of CAT,POD,SOD, GST, AchE enzyme activity of Sogatella furcifera adults and agingnymph,except that transgenic rice with a cry1Ab gene from Bacillus thuringiensis had a significant inhibitory effect on AchE enzyme activity of Sogatella furciferaaging nymph.There were significant differences of Bt toxin protein content of Sogatellafurcifera fed on transgenic rice with a cry1Ab gene from Bacillus thuringiensis amongdifferent instars and among different generations. Bt toxin protein could be detectedfrom Sogatella furcifera nymph and adults fed on transgenic rice with a cry1Ab genefrom Bacillus thuringiensis, but Bt toxin protein content was not enriched with thegrowth of age and the passing of generations. The Bt toxin protein content ofSogatella furcifera showed downward trend in general with the growth of age, and Bttoxin protein content of young nymph higher than that of aging nymph. In addition,the Bt toxin protein content of Sogatella furcifera nymph and adults fed on transgenicrice with a cry1Ab gene from Bacillus thuringiensis existed fluctuation phenomenonfrom the first generation to the third generation.