Effects Of Transgenic Cry1Ab Rice On Insecticide Susceptibility And Activity Of Metabolic Enzymes In Nilaparvata Lugens (St(?)l) And Sogatella Furcifera (Horváth)

Effects of transgenic cry1Ab gene rice, KMD1 and KMD2 on susceptibility and activity of metabolic enzymes in brown planthopper, Nilaparvata lugens and white-backed planthopper, Sogatella furcifera were studied through comparison with those on non-transgenic parental rice variety, Xiushui11 in the paper.1. Effects of transgenic crylAb gene rice on insecticide susceptibility and activity of metabolic enzymes in different populations of N. lugens fed on Bt rice.For all five insecticides tested, there were no significant differences in LD50 values and their generational variation among N. lugens fed on KMD1, KMD2, and Xiushui 11 continuously in the laboratory. The same results were found in the populations of N. lugens collected from rice paddy fields planted with KMD1, KMD2 and Xiushui11 in Hangzhou and Fuyang. For tested activities of detoxification enzymes (esterase and glutathione S-transferase), target enzyme (acetylcholinesterase), and protective enzymes (SOD, CAT and POD), there were no significant differences among different populations of N. lugens either fed on KMD1, KMD2, and Xiushui11 for generations in the laboratory or collected from the rice fields. The content of Bt protein, CrylAb was relatively stable in different populations of N. lugens fed on Bt rice, though it was higher in N. lugens fed on KMD2.2. Effects of transgenic crylAb gene rice on fipronil-resistance and activity of metabolic enzymes in fipronil-resistant strain of N. lugens fed on Bt rice continually in the laboratory.There were no significant differences in LD50 values of fipronil and their generational variation in fipronil-resistant strain of N. lugens fed on KMD1, KMD2, and Xiushui 11 continuously in the laboratory. There were no significant differences in activities of six metabolic enzymes in fipronil-resistant strain of N. lugens fed on KMD1, KMD2, and Xiushui 11 continuously in the laboratory. The generational variation in fipronil-susceptibility and activity of metabolic enzymes of fipronil-resistant strain of N. lugens were similar with those of fipronil-susceptible strain after feeding on KMD1, KMD2, and Xiushui 11 continuously in the laboratory, respectively.3. Effects of transgenic crylAb gene rice on malathion-resistance and activity of metabolic enzymes in malathion-resistant and-susceptible strains of S. furcifera fed on Bt rice continuously in the laboratory.There were no significant differences in LD50 values of malathion and their generational variation in malathion-susceptible strain of S. furcifera fed on KMD1, KMD2, and Xiushui 11 continuously in the laboratory. LD50 values of malathion in malathion-resistant strain of N. lugens fed on KMD2 continuously in the laboratory were significant lower than those fed on Xiushui11, while the differences in LD50 values to malathion and their generational variation in malathion-resistant strain of S. furcifera fed on KMD1 and Xiushui 11 were not significant. There were no significant differences in enzyme activity of three metabolic enzymes of malathion-resistant and-susceptible strains of S. furcifera fed on KMD1, KMD2, and Xiushui 11 continuously in the laboratory, except that the activity of acetylcholinesterase of the malathion-suseptable strain fed on KMD1 and KMD2 for five generations were significant lower than those of S. furcifera fed on Xiushui 11.