Detection Of Progesterone Receptor And Cloning Of Partial Genes In The Mesenteric Ganglion Of Female Goat

IMG is a prevertebral ganglion, issuing the hypogastric nerve which is major source ofsympathetic nerve dominating the uterus (Owman and Sjoberg1966; Kihara and de Groat1997; Labombarda, Guennoun et al.2000) and sympathetic nerve that regulate immunefunction of the uterine.In order to detect the reactiveness of IMG to the progesterone,wedetect the expression of progesterone receptor in the IMG of famele goat by using theImmunohistochemistry, RT-PCR and Western Blot. And the direct performance ofreactiveness of neuvous system to hormone is neurons, so the primary culture for neuron isvery important to the application and research of hormone in the neuvous sysatem. The mainresults obtained in this study are as follows:1. The results of PR stained by immunohistochemistry SP method in IMG of femalegoats showed brown immunoreactive products of PR distributed in neurons, sertoli cells,endothelial cells, schwann cells and other cells, and in the intravascular between neurons.2. The goat progesterone receptor was amplified by PCR and the primers were designedby using bovine progesterone receptor as a template in this experiment. The gPR mRNA wasdetected by PT-PCR. The PCR reactions yield a product at341bp.3. The PCR product was confirmed by DNA sequencing and partial of the female goat'sprogesterone receptor gene was cloned. Then we predicted the protein function of this genefragment.4. Western blot analysis demonstrated the presence of PR protein in the female goat IMG.Two immunoreactive bands of the size expected for gPR (about118kDa and56kDa) weredetected in the female goat IMG.5. The DRG was isolated and cultured with tissue culture method using both serum andserum-free medium.6. The neuro cell surface marker was detected with immunofluorescence and RT-PCR.