Study On Allelopathic Activity Of Energy Grass Chicory

Biomass energy is a kind of high efficient and clean energy, which will become theimportant part of future energy. Chicory (Cichorium intybus) is a perennial Asteraceae energyplant accumulating energy in the form of inulin fructan, which not only has high feedingvalue, but also as an alternative energy source plants are concerned. The complexity anddiversity of chemical composition of Compositae plant are in the first, and they have a lot ofthe biochemical substances. Plant allelopathy in insecticidal, herbicidal, plant protection andecological restoration, has an important role. It can also explain the nature of interactionsbetween plants, but also for reasonable arrangement in planting grass and rotation provideimportant theoretical basis. Therefore, the allelopathic research has become a hot topic in theplant field of biological science. The experiment one was orthogonally designed toincorporate three factors at three levels using the L9(34) orthogonal matrix. It contained threedifferent target species (F. arundinacea, T. repens and M. sativa), three nitrogen treatments(0.5N,1N and2N) and three pH treatments (pH5.5,pH7and pH8.5). There were total of9treatments with three repetitions and comprised of27pots. Test determination contains twoparts. Test one under the treatment of nitrogen and pH interaction determined the donor plantcontent of chlorophyll and malondialdehyde (MDA); catalase (CAT), peroxidase (POD),superoxide dismutase (SOD) activity changeï¼›and the chloroplasts, MDA, soluble sugarcontent of receptor plant with the treatment of different concentrations of chicory osmoticliquid. Test two measured seed germination rate, germination potential, germination index,vigor index, root length and stem length of chicory, tall fescue, clover and alfalfa seedthrough the treatment of chicory extract. And leaf and root extract allelopathic substancecontent related were determined with liquid chromatography. Through those experiments, thequalitative and quantitative allelopathic activity of chicory was systematically studied.The received main results of experiments are as follows:1. Chlorophyll and MDA content were no significant change, but POD and SOD activityrose significantly with different nitrogen concentrations; chlorophyll but MDA content wassignificant change, and activity of POD and SOD rose significantly with different pH values;interaction of nitrogen and pH had significant effect on chicory growth; chlorophyll contentwere greatest with65.305mg/L N and pH5.5, and increased with N applied; MDA content were greatest with261.122mg/L N and pH5.5, and almost no chance when only N or pHstress; POD and SOD activity were highest with261.122mg/L N and pH8.5, and hadsignificant change when only N or pH stress.2. Aqueous extract of chicory root have certain influence on herb seed germination andgrowth of the seedling, namely, the inhibition of germination index, vigor index, seedlingroot and bud growth strengthened with an increase of aqueous extract concentration; seedgermination of festuca suffered the strongest inhibition effect with the same extractconcentration. Different plant seeding roots and stems have different sensitivity to aqueousextract; seeding root and stem of festuca is extraordinary sensitive to aqueous extract, whichinhibition rate reached39.96%and38.83%in50.0g/L extract concentration.3. The nitrogen supply and pH level were located at rectangular area defined by149to168mg/l nitrogen supply combining4.95to7.0pH value and point located at nitrogensupply177mg/l, pH6.33when they were in equivalent coupling effects; whereas theinhibitory effects of equivalent coupling nitrogen supply and pH level were located atrectangular area defined by125to131mg/l nitrogen supply combining6.71to6.88pH valueand two points respectively located at nitrogen supply180mg/l with pH6.38and nitrogensupply166mg/l with pH7.59.4. The chance of pH and nitrogen would affect allelochemical secretion of chicory.Aqueous extracts of chicory roots and leaf accompanied with treatment at different soil pHvalues and nitrogen concentrations influenced its germination, growth and the activity ofantioxidant enzyme.5. The phenolics contents of chicory root and leaf aqueous extracts were1.04g/Kg and0.44g/Kg on average, respectively.