The Production And Control Srtategy Of Cost In Tissue Culture Of Gisela No.5

Large cherry is the first ripe fruit in northern of china, so it enjoys the reputation ofbeing a "spring the fruit first". The stock is one of the key to the development of cherryindustry.In this research, we aimed to learn methods of breeding the cherry rootstock plantlets,find out problems and propose cost control method by accounting for the costs of production,accumulate the knowledge of cultivation techniques of virus-free,dwarf and strong resistantcherry, and lay the foundation for further guidance of the production of cherry plantlets.In the investigation, firstly, we screened the optimum medium for the Gisela5. Withconsulting a lot of data, we proposed and tested5programs for the proliferation medium, andfinally elected a relatively good one, which was MS+IBA0.2mg/L+6-BA0.5mg/L；Forthe rooting medium, we conducted six different treatments,and selected the best rootingmedium components of1/2MS+IBA0.5mg/L+NAA0.5mg/L+Ac0.5g/L.Because the tissue culture hardening transplanting survival rate has enormous impact onthe cost of tissue culture, the factors of affecting tissue culture hardening was investigated.We conducted a meticulous investigation and the actual operation From the tissue cultureseedling emergency time, the punch adapted to the operation of the external environment andthe specific operation of moving to plug, to most important factors of the hardening—thestrict management of the temperature,moisture,ventilation,lighting and the length of theborder, as well as the precautions when transplanting, thus capturing the method ofcontrolling various aspects and factors of the tissue culture during domestication.The production costs of the tissue culture primarily consisted of two main parts: indoordirect production costs, and indoor/outdoor depreciation of fixed assets. The indoor directproduction costs included the cost of medium, utilities, labor and other supplies. Wecalculated the cost of each plant tissue culture was2,003yuan, compared to the market priceof10-12yuan pur strain of the current Gisela5tissue culture, its economic efficiency wasbetter.Through the in-depth investigation and accounting of tissue culture production costs,we put forward a number of recommendations for the cost control measures of tissue culture,including reducing the amount of agar and sugar, shortening the training period and increasing the density of inoculation appropriately, taking effective measures to reduce thecontamination rate, strengthening the management of domesticated hardening to improve thesurvival rate of transplantm rationalizing production, reducing waste, changing cultureconditions and so on.