Studies On The Tissue Culture And Factory Propagation On The Cost Control Of Two Species Of Clematis

Plants of Clematis are perennial vine.They are internationally famous luxury ornamental flowers.Its coverage and growth rate are outstanding.However,the survival rate of this genus is low in the conventional reproduction methods,and the survival rate of cuttings and layers is also low in vegetative propagation,resulting in high production costs,restricting the promotion and development of clematis.In this study,tissue culture was used to explore.Clematis 'Blekitny Aniol'('Lan Tian Shi')and 'Rooguchi'('Zi Ling Dang')as research objects,through stem buds and callus induction pathways,Optimization of methods for tissue culture of different species,establishment of regeneration system,and search for appropriate clematis control the cost of production mode.calculation and comparision,establish a suitable industrialized nursery system of rapid propagation,provides feasibility for the practical application and promotion of gardens.The results of the studies are as follows:1.The establishment of 'Blekitny Aniol' tissue culture and regeneration systemEstablishment of rapid propagation system with bud stemsThe optimal disinfection time is 7 min for stem segments and nodal segments,and 5 min for leaves.The medium for induction of lateral buds is MS basic medium;the initial medium of axillary buds germination is MS+1 mg/L 6-BA+0.05 mg/L NAA;Proliferation medium:1/2 MS+3 mg/L 6-BA+0.01 mg/L NAA;strong seedling medium:MS+0.1 mg/L TDZ+0.05 mg/L NAA,exchange used to increase the growth rate of tissue culture seedlings;suitable rooting medium:1/2MS + 0.05 mg/L NAA,rooting rate 70.22%;the transplanting substrate for vermiculite,perlite and garden soil with a volume ratio of 1:1:2,the survival rate reach 89.27%,and plants growth are strong.Establishment of callus differentiation adventitious buds systemThe appropriate medium for inducing stem callus:MS+2 mg/L 6-BA +0.01 mg/L NAA,induction rate is 78.33%;the suitable medium for inducing leaf callus:MS+2 mg/L 6-BA+0.05 mg/L 2,4-D,induction rate is 92.0%;suitable medium for callus proliferation:MS+0.1 mg/L TDZ+0.01 mg/L NAA,proliferation multiple is 3.47;the suitable medium for callus differentiation is 1/2 MS+0.15 mg/L TDZ,the differentiation rate is 63.89%,the average number of adventitious buds is up to 11,and the growth status are good.2.The establishment of 'Rooguchi' tissue culture and regeneration systemEstablishment of rapid propagation system with bud stemsThe optimal disinfection time is 7 min for and nodal segments,and 5 min for the leaves and stems segments.The medium type for the induction of lateral buds are MS and 1/2 MS,they had no significant difference in the induction of axillary buds.among which the group seedlings in MS medium are thick and strong,and the growth are better.the appropriate medium for the initial stage of axillary buds:MS+1 mg/L 6-BA +0.1 mg/L NAA;the suitable medium for axillary bud proliferation:MS+0.01 mg/L TDZ+0.1 mg/L NAA;Adding 0.5 g/L,activated carbon to this medium can effectively inhibit the browning of phenomenon during the subculture;the appropriate medium combination for rooting is:MS+0.01 mg/L 2,4-D+1 mg/L 6-BA,the rooting rate is 63.33%;the transplanted substrate for vermiculite,perlite,garden soil with a volume ratio of 1:1:3,and the survival rate reach 76.93%.Plants growth are better.Establishment of callus differentiation adventitious buds systemThe optimal medium for inducing stem callus:MS+1 mg/L 6-BA +0.1 mg/L NAA,induction rate is 83.33%;suitable medium for inducing leaf callus:MS +0.5 mg/L 6-BA+0.05 mg/L NAA,induction rate is 68.33%;suitable medium for callus proliferation:MS+0.05 mg/L NAA+2 mg/L 6-BA+0.5 mg/L KT,callus of growth rate are faster and good,and the proliferation ratio is 3.45.The appropriate medium for callus differentiation is MS+3 mg/L 6-BA+0.01 mg/L 2,4-D,and the differentiation rate is 41.67%.3.Low-cost study of Clematis florida tissue culture seedlings:The glass bottle replaces the flask as culture vessel,the tap water replaces the distilled water,the white sugar replaces the sucrose as carbon source.The optimum concentration of white sugar for'Blekitny Aniol' growth is 20 g/L,and the amount of agar is 5 g/L.The optimal concentration of white sugar for 'Rooguchi' is 30g/L,and the amount of agar concentration is 6g/L.In the rooting stage,vermiculite and sand can be used instead of agar.The survival rate of 'Blekitny Aniol' up to 90%when using the vermiculiteand to culture Clemtis florida.The survival rate of 'Rooguchi' up to 87.67%when using the sand to culture Clematis florida.Without considering the costs of labor,equipment,and management,the cost of 'Blekitny Aniol' can be reduced to 26.54%of the original cost,and the cost of 'Rooguchi' can be reduced to 33.13%of the original cost during the same culture of 75 days.The cost margin of the reduction is positively related to the cultivation time.