Construction Of The Bioreactor System On Protocorm-like Bodies Of Dendrobium Officinale

Protocorm-like bodys of Dendrobium officinale were selected as explants.The factors whichaffect protocorm-like bodys biomass, polysaccharide and alkaloid accumulation including lightintensity and culture time were tested; While exploring the conditions of Dendrobium officinaleprotocorm-like bodys bioreactor culture;the relationship between medium composition changesand protocorm-like bodys growth,polysaccharide accumulation were also made.The results wereas follows:1.The results indicated that light intensity at2000Lx for40days was the most effective inincreasing growth and the maximum biomass was0.74g. In this condition, PLBs can keep thestrong split. the maximum polysaccharide Percentage content was16.88%on incubating30daysand Light intensity2000Lx.Light intensity at500Lx for30days was beneficial to alkaloidpercentage content,the maximum is0.028%.2.Results showed that1/2MS medium supplemented with1.0mgï¹’L^-1NAA,50gï¹’L^-1coconutmilk, and30g·L^-1sucrose,6.0pH, was benefit for the proliferation and polysaccharideaccumulation of the PLBs. The culture conditions such as Inoculation with40g PLBs in1L liquidmedium, using15μm pore porous nozzle, aeration volumes by0.10cc min-1and0.05cc min-1used alternately could improve the PLBs growth and polysaccharide contents.3.The fresh mass and dry mass of Dendrobium officinale reached the maximum for36⁴2d.and the content of Polysaccharide alkaloid was the highest (reached24.03%and0.056%) in42dand36d; With the prolonged incubation time at the culture period for36d, the sucroseconcentration in the liquid medium was converted into glucose and fructose, and it dropped to2.0mgï¹’ml-1after20d, but the content of Glucose and fructose reached the highest on the18d andthen decreased. At the same time, by measuring we could find the content of elements, such as N,P,K,Mg,Ca,Zn,Cu,Mn,B, and so on, decreased, and which P, Mg, Ca, of Zn, Mn has beenwidely used.4. Inoculation with40g PLBs in1liter of liquid medium was most suitable for PLBsproliferation and polysaccharide accumulation. spherical bioreactor can be more effective thanother shape bioreactor to promote PLBs proliferation and polysaccharide accumulation; the best treatment of aeration volumes was2.0cc min-1for bioreactor culture. In cultivation process, due tothe consumption of large quantities of nutrients, feeding and refueling midium can be significantlystimulate PLBs proliferation and polysaccharide contents。...