The Tea Endophytic Fungi Secondary Metabolite Detection And Analysis

This paper mainly treats tea tree's endophytic fungi in the southern Henan province as the research object. In the alternate seasons of the year, we use different culture medium on the seasonal isolation and identification of endophytic fungi in each season, finding that there are totally27species in16genera belonging to9families identified, of which Colletotrichum, Macophoma, Phomopsis, the distribution of these3genera of endophytic fungi existing in various seasons with higher separation rate, as advantage endophytic fungi. Research shows that in different seasons, types and volume of tea tree endophytic fungi is different:the most in spring, the least in summer; types and volume of the endophytic fungi in turn trend to increase in summer, fall, winter and spring.Using different methods and means, tests and analyses are made in the tea polyphenols, polysaccharides and grease compounds of the tea tree endophytic fungi secondary metabolites.(1)Tea polyphenolic secondary metabolites are detected by thin layer chromatography, tartaric acid iron colorimetric method and the method of capillary electrophoresis. Three methods detection results are consistent, namely whether fermented liquid or hyphae, they can both produce tea polyphenols, meanwhile it also proves the viewpoint that tea tree's endophytic fungi can produce the same or similar secondary metabolites with host plants;(2) By Fehling reagent on the tea leaf tissue of endophytic fungi in seven target strains of Polysaccharides material detection, each target strains liquid produces brick red precipitate, illustrating that the tea endophytic fungi in the growth and development process in the extracellular polysaccharides can produce secondary metabolites of this trait, which is universal, and find that in the liquor of secondary metabolites of the strain QJ-Mac, sugar content average was0.00137mg/mL;(3) Microscopic morphological observation is operated through480strains of tea tree oil of endophytic fungi characters. The results show that371strains are oil-producing, accounting for77.3%of the total number of strains, of which69strains are high-yield with high oil, accounting for18.6%, which proves that most of the species can produce oil and tea tree oil endophytic fungi is closely related to fungi type; and yielding oil intensively exists in fungus Phomopsis and Fusarium fungal strains.