Proteomics Analysis Of Different Genotypic Rice Responding To Low Phosphorus Stress

As one of essential macronutrients for plant growth, phosphorus (P) is involved in almost all of plant life activities. Phosphorus deficiency in soil is one of the major abiotio stresses that limit plant growth and crop productivity worldwide. Rice is one of the world's major food crops, but also to monocotyledonous species. Proteomics approach to study the rice to phosphorus stress response mechanism, further study on tolerance mechanisms comprehensively reveal the genetic basis of use by the phosphorus nutrition of rice, is of great significance to explorephosphorus response genes, proteins and metabolites.In this study, we choose IR74(high P-absorption efficiency) and IR64(low P-absorption efficiency) as the materials to investigate phosphorus stress resistance mechanism. The total proteins in the root and leaves are obtained respectively of IR74and IR64at low P stress after1hours,3hours,6hours,1days,3days,6days. All the proteins are separated by two-dimensional gel electrophoresis (2-DE) to reveal rice protein response to low phosphorus stress response and dynamic control process.The results obtained are summarized as follows:1. The differentially expressed of root proteins in different P efficiencies rice uptake efficiency genotypeDifferential expression profile of P-dificiency responsive protein between IR74and IR64is constructed by using2-D technology. As an object of study,we choose that in the culture of each period of full-phosphorus have no significant change, but low phosphorus culture the two phosphorus efficiency between culture and total phosphorus there are significant changes in rice root protein. It is found that26proteins of IR74and25proteins of IR64expressed differently.Twenty of them is found in both IR74and IR64expressed differentially in the different treatment time courses significantly. In view of these protein response to phosphorus stress in two rice genotypes in the amount of the difference, that they are low-phosphorus-related protein induced.31root proteins are detected by LC-MS and database searching,27of them are identified. According to their function in plants, those proteins are divided into4categories:4proteins in transcriptional regulation,10proteins in metabolism,3proteins in cell growth,10proteins in cellular defense.2. The differentially expressed of leaf proteins in different P efficiencies rice uptake efficiency genotypeThe results show that22proteins of IR74and19proteins of IR64expressed differently.14of them is found expressed differentially in both1R74and IR64.27of leaf proteins are detected by LC-MS and database searching,25of them are identified. According to their function in plants, those proteins are divided into four categories:7proteins in photosynthesis,8proteins in metabolism,7proteins in Stress responses,3proteins in other processes...