Differential Protepmics On Multi-ovary And Mono-ovary Trait Of Wheat

Proteins are the main elements in a majority of processes in whole organism life,mostof the processes are completed by participation of proteins. Studying proteins is an effectivestrategy to understand the complex biological issue of the relationship between genotype andphenotype and to further reveal the essence of the life phenomenon.It is essencial to researchabout interaction and function of proteins for the sake of answering the life law.Wheat is the most important crop which is widely planted in the world. to increase cropyield and improve quality in wheat. It is effective to use heterosis in crop breeding byincreasing hybrid seed production and selecting crosses with high heterosis To make thewheat heterosis better used in agriculture, we are trying to take the study about the particularmulti-ovary wheat into wheat heterosis research in order to enhance coefficient of wheatbreeding seed,enduce seed production cost, and promote large area of hybrid wheat.Wheat with multi-ovary trait is a new-found special germplasm, specifically expressedwith2-3pistils which can develop into seed commonly and three stamens per flower.It has theadvantages of stable seed-setting and multiple grains which benefit for improving propagationcoefficient and cutting seed production cost.Wheat with multi-ovary trait can effectivelyimprove wheat breeding coefficient,so it is considered as an important germplasm inutilization of wheat heterosis. In this study, further explorations about the reasons for theformation of multi-ovary trait and the application of this particular trait in hybrid wheatbreeding research were done.The differential proteins of panicles at tetrad stage weredetected between the mono-ovary line77(2) and its near-isogenic multi-ovary line Mu77(2)using IEF/SDS-PAGE combine LC-MS/MS method. The following achievements wereobtained.1.Approximately450protein spots were observed in the2-DE gels within the area of pH4–7and molecular weight14.4–97.4kD, of which30were identified as differential proteinspots with at least double expression levels and99%statistical significance. Ten specificprotein spots with differential expressions were then analyzed using LC-MS/MS method.2.Analysised by LC-MS/MS method,the result showed that specific protein spots(S1~S10)are glycine-rich RNA-binding protein,SGT1-1,HSP70,high mobility groupprotein,cytochrome c oxidase subunit,The Small Subunit80s Ribosomal Proteins, HMG-I/Y protein,glutathione transferase,fructose-bisphosphate aldolase,unnamed protein.3.According to the research about the differences of characteristics and functions, thedifferential proteins in organism can be divided into the following categories.Proteins relatedto energy supply and metabolism include fructose1,6-bisphosphate,cytochrome c oxidaseenzyme (COX). Plant stress-related proteins are SGT1protein and glycine-rich RNA-bindingprotein (GRP). Proteins involved in DNA replication, transcription and repair processes arehigh mobility group protein and HMG-I/Y.80S ribosomal small subunit is involved inprotein synthesis.Cell defense-related protein is heat shock protein70(HSP70).Glutathioneglutathione transferase play an essencial role in communication and signaltransduction of cells.4.The formation of multi-ovary trait in wheat, may result from multiple factors. Theseproteins are involved in the regulation of DNA transcription, protein translation, energymetabolism, signal ransduction, plants defense, and other resistance function, and might berelated to the formation of multi-ovary in wheat.