The Effect Of The Prevention With Pseudomonas Aeruginosa Injection On Sepsis Rats

Mortality rate of sepsis caused by gram-negative bacterium infection in clinical is very high, although people has spent a lot of energy and money on the research of sepsis, mortality rate of sepsis still remained no improved. In recent years, people found TLR4 play a very important role in the immune response of gram-negative bacterium infection and systemic inflammatory response syndrome (SIRS) of sepsis, so how to adjust the activity of TLR4, strengthen the immune function, to control the G-bacteria infectious diseases and to avoid SIRS became research hot spot today.Pseudomonas aeruginosa injection (PA-MSHA injection) is a kind of Pseudomonas aeruginosa Mannose-sensitive hemagglutination inactivated vaccine made through genetic engineering technology. So it can activate G- bacterial pathogens related molecular mode receptor TLR4 as G- bacterial pathogens related molecular mode, thus effectively activate the body's immune cells, strengthen the body's immune defenses, it has been widely used in the treatment of various malignant tumor and infectious disease. Firstly, we observe the survival rate of rats with the preventive intervention of different doses and time pseudomonas aeruginosa injection after CLP operation. Secondly we detect the levels of TLR4 and inflammation factors such as TNF-a, IL-1, IL-4, IL-6, IL-10 in tissue and blood samples of the experimental groups significantly different in survival rate and control groups in different times. The paper also discussed the relationship among the survival rate of rats after CLP operation, immune level, inflammation factors and TLR4 activity state.Obejective:1,To evaluation the effect of Pseudomonas aeruginosa injection preventative intervention on the survival of the rats during the 7 days after CLP operation.2,To discuss the relationship among the survival rate of rats after CLP operation, immune level, inflammation factors and TLR4 activity state. Methods:S-D male rats were divided into 5 experimental group A,B,C,D,E, and 1 control group at random,12 rats each group. The intervention of each experimental group are respectively:A(PA-MSHA injection 0.25 ml S.C qod for 8 days), B(PA-MSHA injection 0.5 ml S.C qod for 8 days), C(PA-MSHA injection 0.125 ml S.C qod for 16 days), D(PA-MSHA injection 0.25 ml S.C qod for 16 days), E(PA-MSHA injection 0.5 ml S.C qod for 16 days), control group(without any intervention).all rats were given CLP operation after intervention. The rats of control group were given CLP operation without any intervention. During the 7 days after CLP operation, all rats'physical activity, clinical score,the number of deaths and the time of deaths were observed and recorded, all rats underwent autopsy after death.TLR4 and inflammation factors such as TNF-a, IL-1, IL-6, IL-4, IL-10 in the blood and tissue samples of survival supreme group, survival lowest group and control group was tested after intervention and 12 hours after CLP operation.Results:1,The survival rate of 6 groups 7 days after operation are respectively:group A 58.3%, group B 91.7%, group C 66.6%,group D 70%, group E 41.7%, control group 42.9%.There are significant statistically differences(P<0.05) between group B and control group, group B and group E, in the survival rate and the survival curves comparison.2,The level of TLR4 and inflammation factors of experimental group after the intervention were both increased compared with control group, the increase of the survival supreme group was very significant, and has statistical difference compare with control group (P<0.05). The level of TLR4 and inflammation factors of experimental group after the CLP operation were both reduced compared with control group, the reduce of the survival lowest group was very significant, and has statistical difference compare with control group (P<0.05)Conclusion:1,The preventive application of PA-MSHA injection can improve the survival rate of S-D rats after CLP operation under certain conditions.2,The survival rate of S-D rats after CLP operation is related with immune level, inflammation factors and the activity state of TLR4 before CLP operation.