Research Of Key Virus Nucleic Acids Microarray Discriminative Detection

In recent years, the infectious diseases which were caused by pathogenic microorganism have brought seriously threat to people's health, lives and social stability, national security and some of the high pathogenicity of pathogenic microorganisms may also used by the criminals or terrorist organizations illegally.There are a few microorganisms which have worldwide effect, much greater impact, such as Sars-corona virus, Bird influenza virus, Swine influenza (H1N1 virus), and hand-foot-mouth disease which is characterized by seasonal outbreak and mainly caused by Entero virus 71, Coxsackie virus A16. Most of the diseases were caused by viruses, which belonged to the microorganism and without cell structure. Many countries in the world have defined the viruses as important pathogenic, and put a lot of manpower, material resources for prevention, control and diagnosis. China Ministry of Health has listed them as key objects to monitor. These pathogenic viruses can be divided into intestinal virus, respiratory viruses, and the nervous system viruse, respectively. Because of these pathogenic viruses have more means of communication, faster propagation speed, its virulence genes can be easily integrated, which most likely caused by a large worldwide outbreak and epidemic.It's premise and key for preventing such large-scale virus outbreak and popular to implement the proper measures, provide scientific guidance, accurately diagnose pathogenic viruses. To guarantee people's lives and health, we must strengthen the monitoring and research detection technology for these viruses. Currently, there are so many methods to detect the viruses, such as classical laboratory culture, electron microscopy, physiological and biochemical detection, fluorescence quantitative PCR, antigen-antibody binding, et al. All above methods have some disadvantages. For instance, time consuming, operation complicated, small flux, poor detection on specificity and accuracy. Therefore, they are hard to take effect immediately for these viruses which have a wide range outbreak, no prediction, no obvious features at early infection. With the rapid development of biotechnology, there are many new technologies, such as chemical analysis, biological sensor technology, nucleic acid sequencing technology, PCR technology were applied to detect some important pathogenic viruses at different levels. Gene chip technology was developed in the late 20th century, it could accurately impact on DNA, RNA and other nucleic acid molecules, meet requirement of analysis a large number of sequences at one time as well and it has became primary identification means for many laboratories research and practical application, also viewed as the main development trend for pathogenic microorganisms diagnosis.Gene chips can accurate and fast detection, screening for multiple species or multiple genes of the same species at a short time and more suitable for rapid, high-throughput screen and identify a large number of pathogenic microorganisms. In this article, we have established a method for specific screening respiratory viruses, parainfluenza virus, influenza virus, enterovirus, Entero virus 71, Coxsackie virus A16, 1,2,3-type poliovirus and other entericviruses by using gene chip technology and multiple PCR technology. In order to accurately detect these viruses, different virus-specific probes were selected to develop gene chip technology.