Expressed Of Visfatin And Retinol Binding Protein 4 In The Non-alcoholic Fatty Liver Tissue Of Rats

Objective:Investigate Visfatin and Retinol-binding protein-4(RBP4) expression in Non-alcoholic Fatty Liver Disease (NAFLD) rat liver. Discuss the relation between inappropriate expression of these two genes and the severity of hepatic steatosis which display the role and significance of these genes in pathogenesis of NAFLD. And lay a foundation for non-alcoholic fatty liver diagnosis and treatment.Methods:After 1 week adaptive feeding,40 adult male SD rats were randomly divided into control group(20) and model (20)group. For control group, the rats were given basic diet and subcutaneous injection of saline 0.2ml/100g weight twice a week; the model group were given high fat diet, while the rats were given 40% CC14 salad oil(0.2ml/100g) subcutaneous injection twice a week. For each group,5 rats were randomly selected at 2,4,6 and 8 weekend. Biochemical indicators of body weight, liver index, and heart blood were detected, including ALT, AST, TC and TG; meanwhile, all the liver tissues had been dehydrated, paraffin fixed and HE stained to observe the fatty degeneration degree of liver cells; by RT-PCR and immunohistochemistry, expression levels of visfatin and RBP4 were confirmed in different period in two groups.Results:Experimental results showed that in comparison with control group, body weight, liver index, ALT, AST, TG and TC of model group were increased gradually in 2 weeks,4 weeks,6 weeks and 8 weeks (p<0.05); HE staining result showed that the severity of vacuolar liver steatosis and inflammation changed along with the modeling time increasing; RT-PCR experiments showed that visfatin and RBP4 expression levels increased (p<0.05) with modeling time increasing; immunohistochemistry experiments showed that for each period, compared with control group, visfatin and RBP4 expression were significantly stronger than control group (p<0.05), and with the modeling time tends to increase (p<0.05).Conclusion:In NAFLD rats liver, visfatin and RBP4 expression levels and rat progression of liver steatosis were positively correlated which suggestted that visfatin and RBP4 play an crucial role in this progress and may be a new thought for diagnosis of NAFLD severity.