The Expression Of Interleukin-17 In Peripheral Blood Of Patients With Rheumatoid Arthritis

Objectives:To detective the expression levels of IL-17(interleukin-17)in the patients with rheumatoid arthritis(RA) in differenst phases, and investigating it's effection in the pathogenesis of RA and the relationship between the levels of IL-17A and the activity of RA,to obtain a new clue to enriching RA therapy.Methods:1,RT-PCR:According to 1987 Revised American Rheumatism Association Critera for classification of Rheumatoid Arthritis,37 subjects'peripheral blood were collected and devieded for 3groups:the activity phase group (n=15), the stable phase group (n=12), the health control group (n=10). Extracted each subjects'total mRNA from peripheral blood monocytes(PBMCs), to measure the expression levels of IL-17mRNA by reverse transcription polym erase chain reaction(RT-PCR), and to investigate the relationships between the levels of IL-17mRNA and the clinical indicators, such as patients'ages, serum titres of rheumatoid factors(RF-IgA, RF-IgG, RF-IgM), and anti-cyclic citrullinated peptide(ACCP), DAS28, as well as the levels of erythrocyte sedimentation rate(ESR) and C reactive protein(CRP).2,ELISA:According to 1987 Revised American Rheumatism Association Critera for classification of Rheumatoid Arthritis,37 subjects'peripheral blood were collected and devieded for 3groups:the activity phase group (n=30), the stable phase group (n=28), the health control group (n=18). The serum levels of IL-17 were measured by ELISA, and also the relationships between several lab indicators and serum levels of IL-17 were investigated. Software SPSS17.0 for dates processing.Results:1,RT-PCR:For dates processing, the 3 groups had equal variances(Levene's Test, P>0.05), and there was significantly difference among the 3 groups(P<0.01, AVAON). Among the 3 groups, compared with the health control group, the expression level of IL-17mRNA of RA activity phase group was significantly higher(P<0.01); And the expression level of IL-17mRNA of RA stable phase group was also higher(P<0.05); but there was no significantly difference between RA activity phase group and stable phase group(P>0.05). To detected the correlation between the expression levels of IL-17mRNA and the clinical indicators with Pearson test, it showed there were only significantly correlation between ESR, CRP and the expression level of IL-17mRNA (P<0.05), but not RF, ACCP, patients'ages,DAS28, tender joints and painful joints(P>0.05).2,ELISA::For dates processing, the 3 groups had unequal variances (Levene's Test, P<0.05),nonparametric Test was used, and there was significantly difference among the 3 groups(P<0.01,Kruskal-Wallis H Test). The serum levels of IL-17: compared with the health control group and RA stable phase group, IL-17 level of RA activity phase group was significantly higher(P<0.01);and the level of RA stable phase group was also higher than that of health control group (P<0.01).Conclusion:1,To detect the expression level of IL-17mRNA by RT-PCR method, it showed that the RA group was significantly higher than the health control group, indicating that IL-17 has some relationship with the pathogenesis of RA;2,Of RA group, it showed positive correlations between the expression level of IL-17mRNA and ESR, CRP, and there were positive correlations between the serum levels and RF-IgA,RF-IgM indicating that IL-17 may take part in exciting RA through inducing inflammatory factors,and the level of IL-17 may used to reflet the activity score of RA,as well as to be a new target of RA therapy;3,There were differences of the results between RT-PCR and ELISA,on the one hand, may had some relationship with experiment opration errors, large sample size was needed,on the other hand, may resulted from group devided errors.