Study Of Relationship Of CCL5 Gene Polymorphism With Pulmonary Tuberculosis In The Henan Han Population

Background and ObjectPulmonary tuberculosis (TB) is a highly contagious disease caused by the Mycobacterium tuberculosis. In recent years, with the rapid development of molecular biology, especially human genomics and proteomics, host susceptibility genes has gained increasing attention in the development of research on tuberculosis.Chemokine is a kind of small molecule secreted protein, which can stimulate leukocyte chemotaxis. It is also a type of cytokines subject to the regulation of chemical inducing factors and cytokine, stimulating cell chemotaxis. Main functions of chemokine 5 (CCL5) include:T cell activation and proliferation and induction, activation and support of other co-stimulation T cell cytokines and mononuclear cells, playing an important role in the immune and inflammation regulation process; T cell activation leads to interleukin-2 receptor expression, cytokine release and T cell proliferation.Research found that in Mycobacterium tuberculosis infected macrophages the expression level of CCL5 changes, which has been proved by biochemical metabolism and immunological studies to have close relations with TB infection. Therefore, CCL5 gene and its polymorphism are getting more and more attention. In the further exploration of the relationship between CCL5 gene polymorphism and TB, this paper use the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques to explore the distribution of the two single nucleotide polymorphisms (SNP) rs2280788:-28C/G and rs2107538:-403G/A in the CCL5 promoter region in the Chinese Han population in Henan Province, as well as the distribution of TB risk factors, in order to investigate the role of the two single nucleotide polymorphisms (SNP) in the CCL5 promoter region in the TB occurring process.MethodsSelection of 241 TB cases is consistent with the inclusion criteria consistent with the PRC Ministry of Health Circular [2008] NO.2, TB diagnostic standard file WS 288-2008.-28 point pathological group are 241 cases and control group 270 cases who are randomly selected healthy individuals from those enrolled in body check-ups over the same period;-403 points pathological group are 239 cases, and control group 270 cases who are randomly selected healthy individuals from those enrolled in body check-ups over the same period.Peripheral venous blood samples were collected.Genomic DNA was extracted from white cells.Using the primers, we performed polymerase chain reaction(PCR) amplification and refined frequence length polymorphism(RFLP). These products were electrophoresed on 2.5.0% agarose gels, and DNA was visualized by ethidium bromide straining.Genotype was estimated by the gene-counting method. All statistical analyses were performed with SPSS 17.0 software. Hardy-weinberg equilibrium was confirmed with the association test. Categorical Parameters were summarized as number (%), and compared with chi-square (X2)-test. The relative risk of genotypes and alleles was described by odds ratios (OR) and 95% confidence intervals (95%CI) test standard a=0.05.Results1. In Han population in Henan, the CCL5 gene rs2280788 polymorphism between the TB group and the control group bears no statistic significance and there was no distinct difference.2. In Han population in Henan, CCL5 gene rs2107538 genotype (AA,AG,GG) and allele (G,A) frequency difference between the TB group and the control group was statistically significant (P<0.05).3. Rs2107538 sites and rs2280788 loci of shape analysis, single. Single size analysis found that C-A significantly higher than that in control group (P<0.05), G-A lower than those of the control group (P<0.05).4. Rs2107538 loci of rs2280788 loci genotype and conduct joint analysis genotypes, CC/AG lower than those of the control group (P<0.05), GC/AA, GC/AA, GC/GG, CC/AA higher than those in the control group (P<0.05).5. CCL5 gene rs2107538 sites and the distribution of rs2280788 sites with similar polymorphism in Spain, there was significant difference with Hong Kong, China.Conclusion1. In Han population in Henan, CCL5 gene rs2107538 locus occurred with a significant TB correlation, which may be a risk factor for TB disease as suggested by statistical analysis.2. In Han population in Henan, CCL5 gene in rs2280788 and rs2280788 locus polymorphism, the mutation rate and other regional and ethnic differences.