Study On Relationship Between Polymorphism Sites Of TIM-4 And Allergic Asthma

Object:To investigate two single nucleotide polymorphism sites of T cells immunoglobulin domain and mucin domain protein4( TIM4 ) and detect their relationship with allergic asthma in a population of Hans from Hubei province of China;to screen novel polymorphisms in exton-2 of TIM-4.Methods:The polymorphisms were detected with polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP). Direct PCR-sequencing method was used to screen novel polymorphisms in exton-2 of TIM-4. The genotype and allele frequencies were calculated and analyzed. Total plasma IgE was detected with chemiluminescence immunoassay by ACS180PLUS.Results1. The genotype frequencies of GG,GA and AA in 8570G>A polymorphism were 0.985, 0.015 and 0 respectively in the healthy population and were 0.931, 0.069 and 0 respectively in the allergic asthma population. There was significant difference in genotype and allele frequencies between the allergic asthma patients and control subjects (P = 0. 030, P = 0. 032).2. No significant difference in levels of Total plasma IgE in different genetypes of asthma patients (P>0.05).3. We haven't detected the polymorphisms of 11515C>A and Thr179Ile(C>T).4. The genotype frequencies of GG,GT and TT in + 8727G>Tpolymorphism were 0.9854, 0.0146 and 0 respectively in the healthy population and were 0.9172, 0.0828 and 0 respectively in the allergic asthma population. There was significant difference in genotype and allele frequencies between the allergic asthma patients and control subjects (P = 0. 013, P = 0. 014).Conclusion:The polymorphisms of 8570G>A may be associated with allergic asthma in the population of Han nationality from Hubei province of China,however, this polymorphism does not influence the level of IgE. In the population of Han nationality from Hubei provine of china, there is a novel Polymorphism site (+8727G>T) in the second exon of Tim-4.this Polymorphism may be associated with allergic asthma in the population of Han nationality from Hubei province of China.