The Expression And Clinical Significance Of Livin And Smac In Pancreatic Carcinoma

The pancreatic carcinoma is one of the most deadly of malignant tumors in recent years, the incidence rate showed a clear upward trend (male in the fourth, women account for fifth place). In view of the pancreatic carcinoma by pancreatic anatomy and biological characteristics and other factors, such as early pancreatic cancer surrounding tissue invasion and distant metastasis, no obvious and specific signs and symptoms, the lack of simple and reliable diagnostic methods, the majority of the surgery patients lost the chance of radical resection, only 2.6% to 9% of patients with surgery. Malignant tumor growth has close relationship with apoptosis.In order to further study the role of Smac and Livin in the pancreatic carcinoma,the expression of Smac and Livin in pancreatic tissues, insulin tumor tissues and normal pancreatic tissues will be examed, and the relationship between in them will be investigated.ObjectiveTo explore the expression of Livin and its relationship with Smac in pancreatic carcinoma tissues, insulin tumor tissues and normal pancreatic tissues. According to the expression of them and the pathologic type of tissue specimen, we study the role of Livin and Smac in the occurrence and development of the pancreatic carcinoma. MethodTissue specimens were collected from the department of hepatobiliary surgery of the First Affiliated Hospital of Zhengzhou University from January 2007 to January 2010 and classified three groups, which were not received chemotherapy and radiotherapy and proved pancreatic carcinoma except insulin tumor tissues and normal liver tissues by pathology biopsy. The expressions of Livin and Smac in 46 cases of pancreatic carcinoma tissues and 15 cases of insulinoma tissues and 14 patients normal pancreatic tissues were examined by using immunohistochemical staining. With statistical method to to explore the relationship between Livin and Smac in pancreatic cancer patients and analysis the relationship between them and the clinical data (gender, age, tumor location, tumor size, lymph node metastasis, the degree of differentiation and the clinical stages).Results1. The expression rate of Livin is significantly higher in pancreatic carcinoma (73.9%) and insulinoma tissues(73.3%)than that in non tumorous pancreatic tissues(14.3%).The expression of Livin was significantly correlated with lymph node metastasis,histopathological grading and clinical stage (P<0.05).2. the expression rate of Smac is significantly higher in insulinoma(100%)and non tumorous pancreatic tissues(92.9%)than that in pancreatic carcinoma tissues (39.1%).The expression of Smac was significantly correlated with lymph node metastasis,histopathological grading,clinical stages and patients'age (P<0.05).3. The expression of Livin and Smac was negative correlation in the pancreatic carcinoma tissues by Spearman correlation analysis (r=-0.47,P<0.05).Conclusions1. The expression of Livin in the pancreatic carcinoma was higher than that in insulin tumor tissues and normal pancreatic tissues. Its expression was correlated with lymph node metastasis, histopathological grading and clinical stage. So Livin may be a very important role in the occurrence and process of the pancreatic carcinoma, which may be a marker for diagnosis and prognosis in the pancreatic carcinoma in future.2. The expression of Smac in insulin tumor tissues and normal pancreatic tissues was higher than that in the pancreatic carcinoma.Its expression was correlated with lymph node metastasis, histopathological grading, clinical stages and patients'age. So the lower expression of Smac was very important role in the occurrence and process of the hepatocellular carcinoma.3. The expression of Livin is high and the expression of Smac is low in pancreatic carcinoma, suggesting that downregulation of Livin gene or upregulation of Smac gene in pancreatic carcinoma may become a new target for biological therapy.