The Clinical Observation And Experimental Research On Hepatocellular Carcinoma Treated With TACE And External Application Of Jian PiJie Du Recipe

Purpose:We carried out a prospective random trial by observing 30 Primary liver cancer patients treated by the Chinese herbal medicine and TACE.And to investigate the regulatory effect of JianPiJieDu recipe on the growth of herp1-6 tumor and the expression of bax,bcl-2 in transplanted herp1-6 tumor of mice.Methods:Clinical research:30 Primary liver cancer patients were randomized into two groups:treatment group and controlled group,with 15 patients in each group. The treatment group has being given jianpijiedu recipe one day post TACE, Combined with some western medicine for symptom relief when necessary.The controlled group has being given western medicine only.Then we analyzed the degree and duration of Hepatocellular Carcinoma post-chemoembolization Syndrome,alleviation of clinical symptoms,tumor response rate,improvement of KPS,side effects of TACE and hepatic function.Experimental research:30 nude mice were randomly divided into 3 group(saline group,5-fu group,Jianpijiedu recipe group),with 10 mice per group,0.2ml herp1-6 tumor cell suspension was inoculated in the right axillary(concentration of 1×107/ml),after 24 hours,the mice were treated respectively by saline for intragastric administration,5-fu for abdominal cavity injectionjianpijieduFang for external application daily.14 days later, the tumor size,spleen index,inhibition rate,and expression of bcl-2 by immunohistochemical detection of tumor tissue were observed.Results:(1)The clinical parts①There were significant differences between the treatment group and the controlled group in Hepatocellular Carcinoma post-chemoembolization Syndrome such as fever, pain, nausea and vomiting.There were also significant differences in the degree of adverse effect of TACE, such as anemia and leucocytopenia.②There, were significant differences between the treatment group and controlled group on the improvement of body common conditions.It shows that the treatment group is better than the controlled group in improvement of common conditions.③here were significant differences between the treatment group and the controlled group in AST, ALT.γ-GT and TB of blood serum one and two weeks post TACE.but these datas in the treatment group were lower than the controlled group,P<0.05.It showed that JianPiJieDuFang can relieve the impairment of Hepatic functions post TACE.④here were significant differences between the treatment group and the controlled group in improving clinical symptoms.It shows that the treatment group is better than controlled group in improving clinical symptoms.⑤In the changes of tumor size, the treatment group has no significant difference, P>0.05,as compared with the controlled group.(2)the experimental partsThe rate of tumor suppression, the spleen index were increased, bcl-2-positive rate was decresed in the JianPiJieDu recipe group.Conelusions:JianPiJieDu recipe is more effective, compared with western medicine only,on treating Hepatocellular Carcinoma post-Chemoembolization Syndrome,as well as improving common conditions, Hepatic functions and clinical symptoms. JianPiJieDu recipe could raise the herpl-6 tumor-bearing mice thymus and spleen indexes,and enhance immune function in mice and reduce bcl-2 expression in tumor tissues.