The Protection Of Yupingfeng Decoction On Immune System And Organ After The Liver Cancer-bearing Mice Treated With Cispaltin

OBJECTIVE:To investigate the protection of Yupingfeng Decoction (YPF) on immune system and organ after the liver cancer-bearing mice treated with cispaltin (DDP)METHODS:Separated primary splenic cells from healthy C57mice and cultured in the nutrient medium that contained the different concentrations of YPF or YPF conbined ConA. The cell proliferation was detected by MTT assay. Established the hepatoma model by injecting 2xlO6 Hepal-6 cells into C57 mice intradermally. Then:1) Evaluated the changes of tumor and immune system of these cancer-bearing mice which treated with different doses of DDP by observing the healthy condition, tumor weight, the spleen index and the thymus index.2) Evaluated the changes of tumor and immune system of these cancer-bearing mice which treated with different doses of YPF by observing the living condition, tumor weight, the spleen index and the thymus index, the activity of splenic cell proliferation in vitro, the percentage of splenic lymphocyte and CD4+n CD8+,CDllb+subgroups.3) The liver cancer-bearing mice were treated with YPF and DDP. And the combinational dose of DDP or YPF was definited by the parameters above-mentioned. Beside these parameters above-mentioned, also measured and analyzed the weight of kidney, liver, stomach and lung, detected the activity of Superoxide Dismutase (SOD), malondialdehyde (MDA), and observed pathologic changes.RESULTS:1) DDP had a dose-dependent effect on inhibiting the weight and the growth of tumor.5mg/kg was the dose of the plateau phase of its inhibition effect, which tumor inhibition rate is 74.45%. DDP also can damage the immune organs (spleen and thymus) in dose-dependent manner, and 2.5mg/kg began shows the damage effect.2) YPF had a dose-dependent effect on inhibiting the growth of tumor, and the inhibition rate of its experiment maximal dose (30g/kg) was 32.14%. YPF could promote spleen cell proliferation in vitro, but had no significant effects on the immune organs (spleen and thymus) and the proportion of CD4+and CD8+T cell, nevertheless, YPF could elevate the proportion of splenic lymphocyte and macrophagocyte.3) The tumor inhibition showed an addition effect when combine DDP (2.5mg/kg) with YPF (25g/kg). This combinational therapy could relieve the damages on immune organs, improve the decrease of splenic lymphocyte and macrophagocyte, and correct the abnormal increase of CD4-and CD8+T cell in the liver-cancer-bearing mice. In addition, the combinational therapy also can protect the organ (kidney, liver, stomach, lung) from the damage that induced by tumor and the therapy of DDP.CONCLUSIONS:YPF has a synergism and attenuation effect on the liver-cancer-bearing mice treated with DDP. YPF can protect the immune system and the organs from the damage that induced by tumor and DDP. The possible mechanism of these effects is that YPF can enhance immune function and has the activity of antioxdant.