| Objective:This study is designed to investigate the relationship between coxsackievirus infection and type 1 diabetes mellitus (T1DM), and observe the change of T lymphocyte subsets in the development of T1DM. The animal model part is designed to investigate the relationship between coxsackievirus B3 infection and diabetes mellitus.Methods:GroupⅠwas compoed of 22 cases of newly-diagnosed T1DM and GroupⅡ30 cases of patients who had suffered from diabetes for some time. GroupⅢwas made up of 30 cases of healthy people. We detected Coxsackievirus RNA by reverse transcription PCR (rtPCR), and measured the change of T-lymphocyte subsets by Flow cytometry.Fifty male Balb/c?? mice were divided into five groups randomly (n=10). Mice in group A, group B, group C, group D and group E were infected with different concentrations of viral and placebo. Fasting glucose and postprandial glucose were measured for detecting diabetes. Iimmunohistochemistry of the pancreas was done after the test.Results:The positive rate of Coxsackie virus RNA in Group I was 55.55%, GroupⅡwas 23.33%, and groupⅢwas 6.67%. Chi-Square Test showed that there were a significant differences among the three groups (P<0.01). Patients with upper respiratory tract infection had a higher positive rate of Coxsackie virus RNA than those without in group I (P<0.05). Compared with the control group, the percentage of CD3, CD4 and CD4/CD8 ratio declined in groupⅠand groupⅡwith significant differences (P<0.01 or P<0.05). CD3 and CD4 increased markedly in groupⅡcompared with that in group I, (P<0.01 and P<0.05). Compared with the control group and CVBRNA negative group, CD3, CD4, CD8 and CD4/CD8 were lower in CVBRNA positive group with significant differences (P<0.01 or P<0.05).Conclusions:The positive rate of Coxsackie virus RNA is higher in newly diagnosed type 1 diabetes compared with known type 1 diabetes. T lymphocyte subtypes in type 1 diabetes changed significantly compared with normal control. |
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