| Objective:This study is designed to investigate the relationship between coxsackievirus infection and type 1 diabetes mellitus (T1DM), and observe the change of T lymphocyte subsets in the development of T1DM. The animal model part is designed to investigate the relationship between coxsackievirus B3 infection and diabetes mellitus.Methods:Groupâ… was compoed of 22 cases of newly-diagnosed T1DM and Groupâ…¡30 cases of patients who had suffered from diabetes for some time. Groupâ…¢was made up of 30 cases of healthy people. We detected Coxsackievirus RNA by reverse transcription PCR (rtPCR), and measured the change of T-lymphocyte subsets by Flow cytometry.Fifty male Balb/c?? mice were divided into five groups randomly (n=10). Mice in group A, group B, group C, group D and group E were infected with different concentrations of viral and placebo. Fasting glucose and postprandial glucose were measured for detecting diabetes. Iimmunohistochemistry of the pancreas was done after the test.Results:The positive rate of Coxsackie virus RNA in Group I was 55.55%, Groupâ…¡was 23.33%, and groupâ…¢was 6.67%. Chi-Square Test showed that there were a significant differences among the three groups (P<0.01). Patients with upper respiratory tract infection had a higher positive rate of Coxsackie virus RNA than those without in group I (P<0.05). Compared with the control group, the percentage of CD3, CD4 and CD4/CD8 ratio declined in groupâ… and groupâ…¡with significant differences (P<0.01 or P<0.05). CD3 and CD4 increased markedly in groupâ…¡compared with that in group I, (P<0.01 and P<0.05). Compared with the control group and CVBRNA negative group, CD3, CD4, CD8 and CD4/CD8 were lower in CVBRNA positive group with significant differences (P<0.01 or P<0.05).Conclusions:The positive rate of Coxsackie virus RNA is higher in newly diagnosed type 1 diabetes compared with known type 1 diabetes. T lymphocyte subtypes in type 1 diabetes changed significantly compared with normal control. |
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