| Purpose: Take the ginseng as the example, the discussion traditional Chinese medicine with imitates the biochemistry extraction process research the significance and feasibility, build the bionic technology platform for extraction.Method: Mainly on the ginseng powder and ultra-fine powder at the chemical properties of ordinary, physical and chemical identification, TLC identification and determination of qualitative comparative study; single factor and orthogonal tests on ginseng ultra-fine powder bionic extraction and purification process was optimized; and to extract the initial establishment of quality standards; uses the hematolysis and most greatly to the dose experiment, to verify the bionic ginseng extract ultrafine drug safety; extract effectiveness screening and sure is xylene-inducedweight loading swimming test, climbing pole experiment, hypoxia test to study of bionic ultrafine ginseng extract against fatigue and hypoxia tolerance; indicators for the evaluation of the cell survival rate, uses the MTT method appraisal the ginseng powder superfine imitates the biochemistry extraction after the cell ultraviolet radiation repair damage ability, by determined that the ginseng ultra-fine powder imitates the biochemistry extraction anti-radiation effect.Result: The ginseng ultra-fine powder and the general contrast research trial result indicated, ultra-fine powder after outward appearance character analysis, powder particles uniform, plant cells broken wall is completely by electron microscopy observation; TLC identification and determination of qualitative results show the total saponin dissolution obviously improved; through single factor and orthogonal test inspection, the determination best technological conditions are: bionic solvent pH value to 2.0, solid-liquid ratio is 1:25, control the temperature of 37℃, 60min stirring extraction; by static adsorption method and factorial experimental design method, determine the best process for the separation and purification: taking the concentration of 0.4g/ml (Equivalent to the amount of crude drug) sample fluid (pH=7) on sample, pre-adsorption 3h, after 5BV eluted with water, carries on with the 3BV 50% ethyl alcohol by the 2BV/h speed of flow, get composition clear extract; superfine powder ginseng extract has hemolysis, within the prescribed dose and drug safety; the extract with the same bionic dose of ginseng extract compared to liquid water, significantly prolonged swimming, climbing pole and the duration of hypoxia, and each treatment group compared with the control group there were significant differences; the ginseng ultra-fine powder imitates the biochemistry extraction to be possible obviously to raise the RSa cell survival percentage, and each treatment group with compare for no treatment group has the obvious difference, RSa cell survival is proportional drug concentration.Conclusion: After the ginseng undergoes the micro smashing, cells broken wall rate is high, the release of active ingredient can be directly, It can improve the dissolution rate; the bionic extraction, be more easily absorbed by the body active ingredients; the D-101 macroporous resin can effectively separated imitation biochemical extract, get composition clear PT extract; the craft simple cost is low, suitable for industrial production; the ginseng ultra-fine powder imitates the biochemistry extraction has obvious anti-weary and bears the oxygen deficit function, the onset is rapid, can improve the bioavailability, and to ultraviolet radiation caused apoptosis useful inhibitory action. The safety and efficacy of extracts from studies prove the feasibility of this method. The development of chemical composition for the ginseng provides the basis. |
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