Preparation Of Different Fragments Of Tremella Polysaccharides,Chemical Constitution And The Attenuaed Effect Of Chemotherapy To Tumor Durg

Purpose: Prepare tremella polysaccharide of different molecule weight, investigate their physiochemical and structural properties and screen polysaccharide of optimum activity which decreases toxicity of antitumor drugs and promotes function of leukocytes.Method: Extraction technology was obtained through orthogonal experiment of tremella polysaccharide. Tremella polysaccharides of different molecular weight were prepared by acid hydrolysis, physiochemical properties of which were investigated through phenol sulfuric acid method, m-hydroxybiphenyl method, Lorry method and BCA method. Saccharide component was determined through HPLC, and methylation and GC-MS were used to confirm glycosidic bond pattern. Conduct intravenous injection of normal mice and the team of WBC reduced mice, and observe toxicity reducing ability of tremella polysaccharide with different molecular weights and branch types on antitumor drugs.Result: Optimum extraction technology drawn from orthogonal experiment is described below. Boil samples with 50 times of water for 3 times, and each time for 3 hours. Tremella polysaccharides of 200,000 Dalton, 30,000 Dalton and 4,000 Dalton were prepared through acid hydrolysis. Physiochemical properties were determined. Molecule of 200,000 Dalton contains 76.5% of neutral saccharides, 19.8% of acid saccharides and 9% of proteins. Molecule of 30,000 Dalton contains 78.2% of neutral saccharides 22.1% of acid saccharides and 6.2% of proteins. Molecule of 4,000 Dalton contains 83.1% of neutral saccharides 22.3% of acid saccharides and 8.2% of proteins. HPLC analysis shows, the above mentioned different molecules are composed of Man, Xyl,GlcA.GC-MS results showed that the fragments of Tremella polysaccharides with different molecular weights are connected to Man 1→3-based chain, and contains a small amount of 1→4 linked Xyl.Mainly by its non-reducing end GlcA composition. It can be concluded from structure-activity analysis and pharmacological actions of tremella polysaccharide that both molecule of 30,000 Dalton and molecule of 4,000 Dalton could promotes leukocytes better when treating antitumor drugs caused decrease of peripheral blood leukocytes.Conclusion: This experiment investigates isolation,hydrolysis,physiochemical properties,structure-activity relationship, and pharmacology of tremella polysaccharide. Sugar fractions of tremella polysaccharide which decreases toxicity of antitumor drugs and promotes leukocyte, were screened. Structure-activity relationship and function mechanism of tremella polysaccharide were summarized, which is of importance to drug development.