The Setup Of Rat Models For Hyperuricemia Renal Damage

Objective:to set up animal models for hyperuricemia damage to kidney and create conditions to further study the mechanism of hyperuricemia renal damage.Methods:Ladenine way:20 male Wistar rats were divided into two groups randomly, control group 10 and model group 10.The rats in model group were given adenine 100mg/kg once a day orally and distilled water was administered orally to rats in the control group.The serum uric acid and creatinine levels were monitored weekly. At the end of the 2nd week,rats were killed and pathological changes were observed.2.potassium oxonate way:20 male Wistar rats were randomly divided into control group and model group. Potassium oxonate 400mg/kg was given orally to rats in model group twice a day and distilled water was administered orally to rats in control group. The serum uric acid and creatinine levels were monitored.At the end of 9th week,all the rats were killed and pathological damages were observed.Results:1.adenine way:the serum creatinine levels in model group were higher than control group, but serum uric acid in both groups had no difference. The kidneys of model group were much larger paler and plenty of white granules deposited on the surface, under light microscope,yellow-brown depositions deposited in the kidney tubules and renal interstitium was swelling and many inflammatory cells infiltrated in the interstition. the serum cretinine rised before hyperuricemia, we can conclude that adenine metabolized to 2,8-dyhydroxyl adenine and damaged kidneys, then serum uric acid rised secondly.2.OXO way:At the end of 1st 2nd 3rd 6th and 9th week, uric acid levels were all higher than those of control group,while there was no difference in serum creatinine in both groups. At the end of 9th week, we found no changes in outward appearances between control and model group, and under light microscope no deposition was seen in the tubules, the epithelial cells were swelling.Conclusions: 1.adenine way: This model didn't suit the study for primary hyperuricemia renal damage.2.OXO way: hyperuricemia renal damage model could be successfully induced by OXO 400mg/kg twice a day orally.