The Plasma Chemerin Level Is Related To Vasculopathy Of Lower Extremities In Type 2 Diabetes Mellitus

ObjectiveVessels complications are main hazards of diabetes mellitus, vasculopathy of lower extremities is the main reason in disabled diabetics. In present, we study that obesity, insulin resistance(IR), adipocytokines, Type 2 Diabetes Mellitus and Vessels complications of Type 2 Diabetes Mellitus has a close relationship and interaction. Many studies have found that TNF-α, IL-6 and so on are important factors in development of vessels complications of diabetes mellitus. Chemerin is a new type of adipocytokines which found in recent years, it has expressed in kidney, liver and other organizations, and also highly expressed in fat cells. Chemerin participate in the immune response and inflammatory reaction, and play a role in fat metabolism. But in Type 2 Diabetes Mellitus patients, Chemerin level changes are disputes, and the relationship between chemerin level and vasculopathy of lower extremities is not clearly. This research analyse the relationship between chemerin level and obesity, insulin resistance(IR) and vasculopathy of lower extremities of Type 2 Diabetes Mellitus. Through measuring the chemerin level changes of different degree of vasculopathy of lower extremities in Type 2 Diabetes Mellitus, and understand the chemerin level changes of diabetics and the effect of chemerin level changes in vasculopathy of lower extremities.MethodsEighty outpatients of T2DM and twenty normal controls were selected as research subjects, age 45 to 70, pathogenesis five to ten years. Under examination of color Doppler ultrasound, T2DM patients were divided into four groups according to degree of vasculopathy of lower extremities:simple type 2 diabetes without vasculopathy of lower extremities which had 16 patients(group DMo), type 2 diabetes with thicked vascular intima of lower extremities had 19 patients(group DM1), type 2 diabetes with ngiosclerosis of lower extremities had 23 patients(grpup DM2), type 2 diabetes with angiostegnosis of lower extremities had 22 patients(group DM3). All subjects had normal blood pressure, and did not have serious heart, liver, kidneys diseases. Group NC include normal healthy without hypertension and diabetes. There is no difference between age and gender. All subjects fasting 10 hours and the next day withdrawal. Measured HbA1c, Insulin, Chemerin respectively. The plasma chemerin, TNF-αand CRP levels of every guoup measured by enzyme linked immunosorbent assay(ELISA), and analyes the relationship between the plasma chemerin, TNF-αand CRP levels and BMI, Body fat ingredients(FTA%), HbA1C and insulin resistance index(HOMA-IR). Data were analysed using the Statistical Package of the SPSS16.0. Discriptive data were given as means±SD(x±s).Continuous variable were tested by analysis of t-test, group comparison were tested by analysis of variance and the relations between the variables were tested by linear correlation.The p-values are two-tailed and a p-value of less than 0.05 being considered to be significant.Results1.Every groups compared, BMI,FAT%,HbA1c(%)å'ŒHOMA-IR of indexes exists remarkable difference. DM1 was higher than DM0(P<0.01). DM2 was higher than DM1 (P<0.01); DM3 was higher than DM1 and DM2 (P<0.01).2.The plasma TNF-aand CRP levels compared among every guoups, DM was higher than NC(P<0.05); Every DM groups compared, DM1, DM2, DM3 was higher than DM0(P<0.05); Every group of Type2 Diabetes Mellitus with vasculopathy of lower extremities compared, DM2 was higher than DM1 (P<0.05), DM3 was higher than DM1 (P<0.05) and DM2 (P<0.05).3.2.The plasma chemerin levels compared among every guoups, DM was higher than NC (P<0.05); Every DM groups compared, DM1, DM2, DM3 was higher than DM0(P<0.05); Every group of Type2 Diabetes Mellitus with vasculopathy of lower extremities compared, DM2 was higher than DM1 (P<0.05), DM3 was higher than DM1(P<0.05) and DM2 (P<0.05). 4. Correlation analysis showed that the plasma TNF-αlevels were positively correlated to BMI (r=0.816), FAT%(r=0.784), HbA1C(r=0.512, HOMA-IR(r=0.776)respectively, (P<0.01).5. Correlation analysis showed that the plasma CRP levels were positively correlated to BMI (r=0.820), FAT%(r=0.727), HbA1C(r=0.543 HOMA-IR(r=0.737)respectively, (P<0.01).6.Correlation analysis showed that the plasma chemerin levels were positively correlated to BMI (r=0.792), FAT%(r=0.717), HbA,C (r=0.418, HOMA-IR(r=0.684)respectively, (P<0.01).7. Correlation analysis showed that the plasma chemerin levels were positively correlated to TNF-α(r=0.847), CRP(r=0.884) respectively, (P<0.01).ConclusionThe plasma chemerin level of Type 2 Diabetes was increased, obesity, body fat percentage, insulin resistance are independent factors in the plasma chemerin level of Type 2 Diabetes which was increased. plasma chemerin levels may be relevanted to the development of vasculopathy of lower extremities in Type 2 Diabetes Mellitus. Chemerin may be one factor of the development vasculopathy of lower extremities in Type 2 Diabetes Mellitus.