The Expression And Significance Of Mismatch Repair Gene HMLH1 And HMSH2 In The Canceration Process From Hepatolithiasis To Cholangiocarcinoma

Objective:To detect the relative content of hMSH2 and hMLH1 in different bile duct cells of hepatolithiasis and cholangiocarcinoma patients,research the expression and significance of hMSH2 and hMLH1 in the process of intrahepatic bile duct carcinogenesis.Methods:To detect the relative content of hMSH2 and hMLH1 in normal intrahepatic bile duct tissues (control group),less than ten years (Calculus group 1) and more than ten years (Calculus group 2) bile duct tissues of hepatolithiasis patients,choledocholithiasis and cholangiocarcinoma tissues(cholangiocarcinoma group) by fluorescence quantitative PCR.Results:The relative content of hMSH2 and hMLH1 is highest in normal group,calculus group is higher than cholangiocarcinoma group,it is significantly different among normal group,calculus group and cholangiocarcinoma group (P<0.05).The relative content of hMSH2 and hMLHl of calculus group 1 is higher than calculus group 2,it is significantly different between two groups(P<0.05). The relative content of hMSH2 and hMLH1 of cholangiocarcinoma group is lower than calculus group 2.Conclusions:The long-term stimulation of intrahepatic bile duct stones can lead to the different hMSH2 and hMLH1 express, and with the extension of intrahepatic bile duct stimulate, the expression is lower. The expression of hMSH2 and hMLH1 in cholangiocarcinoma group is lowest, prompt that hMLH1, hMSH2 is intrahepatic bile duct with long-term inflammatory stimulation cause bile duct cancer is one of the important factors.